The GTPase domain of Galphao contributes to the functional interaction of Galphao with the promyelocytic leukemia zinc finger protein

Go, one of the most abundant heterotrimeric G proteins in the brain, is classified as a member of the Gi/Go family based on its homology to Gi proteins. Recently, we identified promyelocytic leukemia zinc finger protein (PLZF) as a candidate downstream effector for the alpha subunit of Go (Gαo). Act...

Full description

Saved in:
Bibliographic Details
Published in:Cellular & molecular biology letters 2009-03, Vol.14 (1), p.46-56
Main Authors: Won, Jung, Ghil, Sung
Format: Article
Language:English
Subjects:
Cell growth
Cell Proliferation
Cyclin A - metabolism
Differentiation
Domain
G protein
GTP Phosphohydrolases - chemistry
GTP-Binding Protein alpha Subunits, Gi-Go - chemistry
GTP-Binding Protein alpha Subunits, Gi-Go - metabolism
Humans
Kruppel-Like Transcription Factors - metabolism
Protein Binding
Protein Structure, Tertiary
Sequence Deletion
Short Communication
Structure-Activity Relationship
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Go, one of the most abundant heterotrimeric G proteins in the brain, is classified as a member of the Gi/Go family based on its homology to Gi proteins. Recently, we identified promyelocytic leukemia zinc finger protein (PLZF) as a candidate downstream effector for the alpha subunit of Go (Gαo). Activated Gαo interacts with PLZF and augments its function as a repressor of transcription and cell growth. G protein-coupled receptor-mediated Gαo activation also enhanced PLZF function. In this study, we determined that the GTPase domain of Gαo contributes to Gαo:PLZF interaction. We also showed that the Gαo GTPase domain is important in modulating the function of PLZF. This data indicates that the GTPase domain of Gαo may be necessary for the functional interaction of Gαo with PLZF.
ISSN:1689-1392
1425-8153
1689-1392
DOI:10.2478/s11658-008-0033-6