Functional conservation of yeast mtTFB despite extensive sequence divergence

Transcription of mtDNA in the yeast S. cerevisiae depends on recognition of a consensus nonanucleotide promoter sequence by mtRNA polymerase acting with a 40-kDa dissociable factor known as mtTFB or Mtflp. mtTFB has been cloned and characterized in S. cerevisiae, but has not been studied in similar...

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Bibliographic Details
Published in:Gene expression 1996-01, Vol.6 (4), p.219-230
Main Authors: Carrodeguas, J.A. (State University of New York at Stony Brook.), Yun, S, Shadel, G.S, Clayton, D.A, Bogenhagen, D.F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
BINDING PROTEINS
BIOLOGICAL DIFFERENCES
CHEMICAL COMPOSITION
CLONACION
CLONAGE
CLONING
COMPLEMENTATION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Conserved Sequence
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DNA, Fungal - chemistry
DNA, Fungal - metabolism
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - metabolism
Evolution, Molecular
Fungal Proteins - genetics
Fungal Proteins - metabolism
genbank/u81619
genbank/u81620
GENE
GENES
GENETICA
GENETICS
GENETIQUE
Kluyveromyces - genetics
Kluyveromyces - metabolism
Kluyveromyces lactis
KLUYVEROMYCES MARXIANUS
KLUYVEROMYCES MARXIANUS VAR. LACTIS
Mini-Review
Mitochondria - metabolism
Mitochondria Mttfb
Mitochondrial Proteins
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Polymerase Chain Reaction
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
Recombinant Proteins - metabolism
RNA POLYMERASE
SACCHAROMYCES
Saccharomyces - genetics
Saccharomyces - metabolism
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Saccharomyces kluyveri
SECUENCIA NUCLEOTIDICA
Sequence Alignment
SEQUENCE NUCLEOTIDIQUE
Sigma Factors
SPECIES DIFFERENCES
STRUCTURAL GENES
TRANSCRIPCION
TRANSCRIPTION
TRANSCRIPTION FACTORS
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
TRANSFERASAS
TRANSFERASE
TRANSFERASES
Yeast Transcription
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Summary:Transcription of mtDNA in the yeast S. cerevisiae depends on recognition of a consensus nonanucleotide promoter sequence by mtRNA polymerase acting with a 40-kDa dissociable factor known as mtTFB or Mtflp. mtTFB has been cloned and characterized in S. cerevisiae, but has not been studied in similar detail in any other organism. Although it is known that mitochondrial transcription in the dairy yeast, Kluyveromyces lactis, initiates within the same consensus promoter sequence used in S. cerevisiae, no previous studies have focused on the proteins involved in transcription initiation in K. lactis. In this article, we report the cloning of mtTFB from K. lactis and from a yeast more closely related to S. cerevisiae, S. kluyveri. Both novel mtTFB genes were able to substitute for the MTF1 gene in S. cerevisiae. Both proteins purified following expression in E. coli were able to support specific transcription initiation in vitro with the S. cerevisiae mtRNA polymerase. The S. kluyveri and K. lactis mtTFB proteins share only 56% and 40% identity with S. cerevisiae mtTFB, respectively. Alignments of the three mtTFB sequences did not reveal any regions larger than 30 amino acids with greater than 60% amino acid identity. In particular, regions proposed to show sequence similarity to bacterial a factors were not more highly conserved than other regions of the mtTFB proteins. All three yeast mtTFB genes lack conventional amino-terminal mitochondrial targeting sequences, suggesting that all three proteins may be imported into mitochondria by the same unusual mechanism reported for S. cerevisiae mtTFB.
ISSN:1052-2166
1555-3884