Autophagy is required for proper meiosis of porcine oocytes maturing in vitro

Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3...

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Bibliographic Details
Published in:Scientific reports 2018-08, Vol.8 (1), p.12581-12, Article 12581
Main Authors: Shen, Xing-Hui, Jin, Yong-Xun, Liang, Shuang, Kwon, Jeong-Woo, Zhu, Ji-Wei, Lei, Lei, Kim, Nam-Hyung
Format: Article
Language:English
Subjects:
Apoptosis
Autophagy
DNA damage
Meiosis
Mitochondria
Mitochondrial DNA
Oocytes
Organelles
Phagocytosis
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Summary:Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44 h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown and treatment with the autophagy inhibitor, LY294002, during maturation of porcine oocytes in vitro impaired polar body extrusion, disturbed mitochondrial function, triggered the DNA damage response, and induced early apoptosis in porcine oocytes. Autophagy inhibition during oocyte maturation also impaired the further developmental potential of porcine oocytes. These results indicate that autophagy is required for the in vitro maturation of porcine oocytes.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-29872-y