Influence of media supplements on inhibition of oxidative browning and bacterial endophytes of Camellia sinensis var. sinensis

Explant oxidative browning and necrosis of Camellia sinensis var. sinensis is a severe problem in tissue culture, often associated with the exuded phenolic compounds and microbial contamination from the explants. In this study, 2-aminoindane-2-phosphonic acid (AIP), an inhibitor of the polyphenol pr...

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Bibliographic Details
Published in:3 Biotech 2018-08, Vol.8 (8), p.356-7, Article 356
Main Authors: Alagarsamy, Karthikeyan, Shamala, Lubobi Ferdinand, Wei, Shu
Format: Article
Language:English
Subjects:
Agriculture
Ammonia
Antibiotics
Bioinformatics
Biomaterials
Biotechnology
Browning
Callus
Camellia sinensis
Cancer Research
Chemistry
Chemistry and Materials Science
Contamination
Culture media
Data processing
Endophytes
Explants
Exudation
Gangrene
Gentamicin
Microbial contamination
Microorganisms
Necrosis
Phenolic compounds
Phenols
Phenylalanine
Protocols and Methods
Stem Cells
Tea
Thidiazuron
Ticarcillin
Tissue culture
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Summary:Explant oxidative browning and necrosis of Camellia sinensis var. sinensis is a severe problem in tissue culture, often associated with the exuded phenolic compounds and microbial contamination from the explants. In this study, 2-aminoindane-2-phosphonic acid (AIP), an inhibitor of the polyphenol production-required enzyme phenylalanine ammonia lyase (PAL), and different antibiotics were tested to control tea explant necrosis and browning. These compounds were supplemented in the regular plant growth medium together with 6-benzylaminopurine and thidiazuron at different concentrations. Our data indicated that application of 2 µM of AIP was able to effectively inhibit callus browning, significantly reduce EGC abundance, and greatly improve callus induction and growth. Moreover, the use of 150 mg/L of timentin and 30 mg/L gentamycin resulted in an effective elimination of the surface and endophytic microbes associated with explants of C. sinensis var. sinensis . Our study revealed that the inhibition of PAL using AIP combined with the two tested antibiotics could open up new doors to control oxidative tissue browning and endophyte contamination in tissue culture for tea genetic manipulation.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-018-1378-9