In vitro biological screening of a critically endangered medicinal plant, Atropa acuminata Royle Ex Lindl of north western Himalaya

Atropa acuminata Royle Ex Lindl ( Atropa acuminata ) under tremendous threat of extinction in its natural habitat. However, the antimicrobial, antileishmanial and anticancer effects of the plant’s extracts have not been reported yet. In the current study, an attempt has been made to evaluate the pha...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2018-07, Vol.8 (1), p.11028-10, Article 11028
Main Authors: Rahman, Khaista, Khan, Shahid Ullah, Fahad, Shah, Shinwari, Zabta Khan, Khan, Dilfaraz, Kamal, Sajid, Ullah, Ikram, Anjum, Syed Ishtiaq, Man, Shad, Khan, Abdul Jamil, Khan, Wasim Ullah, Khan, Muhammad Hafeez Ullah, Jan, Mehmood, Adnan, Muhammad, Noor, Muhammad
Format: Article
Language:English
Subjects:
631/1647
631/449
Acetone
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-Infective Agents - chemistry
Anti-Infective Agents - pharmacology
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Artemia - drug effects
Atropa
Atropa - chemistry
Brines
Cancer
Cytotoxicity
Endangered plants
Endangered Species
Ethanol - chemistry
Humanities and Social Sciences
Kinases
Leaves
Leishmania - drug effects
Medicinal plants
Methanol - chemistry
Microbial Sensitivity Tests
multidisciplinary
n-Hexane
Natural products
Plant extracts
Plant Extracts - chemistry
Plant Leaves - chemistry
Plant Roots - chemistry
Plants, Medicinal - chemistry
Protein kinase
Roots
Science
Science (multidisciplinary)
Solvents
Solvents - chemistry
Threatened species
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Atropa acuminata Royle Ex Lindl ( Atropa acuminata ) under tremendous threat of extinction in its natural habitat. However, the antimicrobial, antileishmanial and anticancer effects of the plant’s extracts have not been reported yet. In the current study, an attempt has been made to evaluate the pharmacological potential of this plant’s extracts against microbes, Leishmania and cancer. The roots, stems and leaves of Atropa acuminata were ground; then, seven different solvents were used alone and in different ratios to prepare crude extracts, which were screened for pharmacological effects. The aqueous, methanolic and ethanolic extracts of all parts carried a broad spectrum of anti-bacterial activities, while no significant activity was observed with combined solvents. Three types of cytotoxicity assays were performed, i.e., haemolytic, brine shrimp and protein kinase assays. The aqueous extract of all the parts showed significant haemolytic activity while n-hexane extracts of roots showed significant activity against brine shrimp. The acetone extracts strongly inhibited protein kinase while the methanolic extracts exhibited significant cytotoxic activity of roots and stem. The anti-leishmanial assays revealed that the methanolic extract of leaves and roots showed significant activity. These findings suggest that this plant could be a potential source of natural product based drugs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-29231-x