Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome

Axonal protein synthesis and degradation are rapidly regulated by extrinsic signals during neural wiring, but the full landscape of proteomic changes remains unknown due to limitations in axon sampling and sensitivity. By combining pulsed stable isotope labeling of amino acids in cell culture with s...

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Bibliographic Details
Published in:	Neuron (Cambridge, Mass.) Mass.), 2018-07, Vol.99 (1), p.29-46.e4
Main Authors:	Cagnetta, Roberta, Frese, Christian K., Shigeoka, Toshiaki, Krijgsveld, Jeroen, Holt, Christine E.
Format:	Article
Language:	eng
Subjects:	Amino acids Animals Ataxia axon axon guidance Axons Axons - drug effects Axons - metabolism Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - pharmacology Cell culture Cells, Cultured chemotropic response Congenital diseases Embryo, Nonmammalian extrinsic cues Gene Expression Regulation growth cone Intellectual disabilities Isotope Labeling local protein synthesis Mass Spectrometry Netrin-1 Netrin-1 - pharmacology neural wiring Neuronal Outgrowth - drug effects Protein biosynthesis Proteins Proteome - drug effects Proteome - metabolism Proteomes Proteomics pSILAC-SP3 Retina retinal ganglion cell Retinal Ganglion Cells - drug effects Retinal Ganglion Cells - metabolism Semaphorin-3A - pharmacology Semaphorins Xenopus laevis
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Summary:	Axonal protein synthesis and degradation are rapidly regulated by extrinsic signals during neural wiring, but the full landscape of proteomic changes remains unknown due to limitations in axon sampling and sensitivity. By combining pulsed stable isotope labeling of amino acids in cell culture with single-pot solid-phase-enhanced sample preparation, we characterized the nascent proteome of isolated retinal axons on an unparalleled rapid timescale (5 min). Our analysis detects 350 basally translated axonal proteins on average, including several linked to neurological disease. Axons stimulated by different cues (Netrin-1, BDNF, Sema3A) show distinct signatures with more than 100 different nascent protein species up- or downregulated within the first 5 min followed by further dynamic remodeling. Switching repulsion to attraction triggers opposite regulation of a subset of common nascent proteins. Our findings thus reveal the rapid remodeling of the axonal proteomic landscape by extrinsic cues and uncover a logic underlying attraction versus repulsion. [Display omitted] •pSILAC-SP3 reveals the newly synthesized axonal proteome within minutes•Extrinsic cues rapidly up- and downregulate large subsets of nascent proteins•Different repulsive cues generate distinct proteomic signatures•Switch from repulsion to attraction elicits opposite changes in nascent proteome Cagnetta et al. establish a highly sensitive proteomics approach to characterize the nascent proteome of a subcellular compartment (axon) on a rapid timescale (minutes). Remarkably, different extrinsic cues trigger distinct signatures of up- and downregulated nascent protein changes within 5 min.
ISSN:	0896-6273 1097-4199