Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies in Haloxylon persicum (Bunge ex Boiss & Buhse): an endangered desert tree species

Haloxylon persicum (Bunge ex Boiss & Buhse), is one of the hardy woody desert shrubs, which is now endangered and/or nearing extinction. Urban landscape development and overgrazing are the major threats for the erosion of this important plant species. For conserving the species, it is critical t...

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Published in:Physiology and molecular biology of plants 2018-07, Vol.24 (4), p.683-692
Main Authors: Kurup, Shyam Sreedhara, Thayale Purayil, Fayas, Alkhaili, Mubarak Mohammed Sultan, Tawfik, Nadia Hassan, Cheruth, Abdul Jaleel, Kabshawi, Maher, Subramaniam, Sreeramanan
Format: Article
Language:English
Subjects:
Banding
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Boron
Calcium
Cell Biology
Desert environments
Deserts
Endangered plants
Endangered species
Erosion
Explants
Haloxylon persicum
Kinetin
Life Sciences
Necrosis
Organogenesis
Parents
Plant Physiology
Plant Sciences
Plant species
Regeneration
Research Article
Shoots
Shrubs
Species extinction
Subculture
Subcultures
Thidiazuron
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Summary:Haloxylon persicum (Bunge ex Boiss & Buhse), is one of the hardy woody desert shrubs, which is now endangered and/or nearing extinction. Urban landscape development and overgrazing are the major threats for the erosion of this important plant species. For conserving the species, it is critical to develop an efficient in vitro regeneration protocol for rapid multiplication of large number of regenerants. Leaf explants, cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) (0.5, 1, 2 µM), showed significant difference in bud sprouting and adventitious shoot induction. The highest shoot bud formation was recorded on MS medium supplemented with 0.5 µM TDZ. Shoot tip necrosis (STN), observed after first subculture of shoot buds in same medium, increased in severity with subculture time. Application of calcium (4 mM) and boron (0.1 mM) in combination with kinetin (10 µM) in the subculture medium significantly reduced the intensity of STN. On an average eight shoots/explant were produced by alleviating this problem. ISSR marker analysis revealed monomorphic banding pattern between progenies and parents, indicating the true to type nature of the clones and its parents.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-018-0532-5