Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes

The compartmentalization of cell-free gene expression systems in liposomes provides an attractive route to the formation of protocells, but these models do not capture the physical (crowded) environment found in living systems. Here, we present a microfluidics-based route to produce monodisperse lip...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2018-06, Vol.140 (24), p.7399-7402
Main Authors: Deng, Nan-Nan, Vibhute, Mahesh A, Zheng, Lifei, Zhao, Hui, Yelleswarapu, Maaruthy, Huck, Wilhelm T. S
Format: Article
Language:English
Subjects:
Artificial Cells - chemistry
Communication
Emulsions - chemistry
Liposomes - chemistry
Membrane Lipids - chemistry
Microfluidics - methods
Poloxamer - chemistry
Polyethylene Glycols - chemistry
Surface-Active Agents - chemistry
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Summary:The compartmentalization of cell-free gene expression systems in liposomes provides an attractive route to the formation of protocells, but these models do not capture the physical (crowded) environment found in living systems. Here, we present a microfluidics-based route to produce monodisperse liposomes that can shrink almost 3 orders of magnitude without compromising their stability. We demonstrate that our strategy is compatible with cell-free gene expression and show increased protein production rates in crowded liposome protocells.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.8b03123