Co‐expression of μ and δ opioid receptors by mouse colonic nociceptors

Background and Purpose To better understand opioid signalling in visceral nociceptors, we examined the expression and selective activation of μ and δ opioid receptors by dorsal root ganglia (DRG) neurons innervating the mouse colon. Experimental Approach DRG neurons projecting to the colon were iden...

Full description

Saved in:
Bibliographic Details
Published in:British journal of pharmacology 2018-07, Vol.175 (13), p.2622-2634
Main Authors: Guerrero‐Alba, Raquel, Valdez‐Morales, Eduardo Emmanuel, Jiménez‐Vargas, Nestor Nivardo, Bron, Romke, Poole, Daniel, Reed, David, Castro, Joel, Campaniello, Melissa, Hughes, Patrick A, Brierley, Stuart M, Bunnett, Nigel, Lomax, Alan E, Vanner, Stephen
Format: Article
Language:English
Subjects:
Action potential
Animals
Calcium antagonists
Calcium channels (voltage-gated)
Calcium currents
Calcium ions
Capacitance
Colon
Colon - metabolism
Dorsal root ganglia
Excitability
Gene Expression Profiling
In Situ Hybridization, Fluorescence
Inflammation
Male
Mice
Mice, Inbred C57BL
Narcotics
Neurons
Nociceptors
Nociceptors - metabolism
Opioid receptors
Pain perception
Receptors, Opioid, delta - biosynthesis
Receptors, Opioid, delta - genetics
Receptors, Opioid, mu - biosynthesis
Receptors, Opioid, mu - genetics
Research Paper
Research Papers
Sensory neurons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background and Purpose To better understand opioid signalling in visceral nociceptors, we examined the expression and selective activation of μ and δ opioid receptors by dorsal root ganglia (DRG) neurons innervating the mouse colon. Experimental Approach DRG neurons projecting to the colon were identified by retrograde tracing. δ receptor‐GFP reporter mice, in situ hybridization, single‐cell RT‐PCR and μ receptor‐specific antibodies were used to characterize expression of μ and δ receptors. Voltage‐gated Ca2+ currents and neuronal excitability were recorded in small diameter nociceptive neurons (capacitance
ISSN:0007-1188
1476-5381
DOI:10.1111/bph.14222