PCR identification of toxic euglenid species Euglena sanguinea

Euglena sanguinea Ehrenberg is the only known species of euglenids which forms toxic blooms causing tangible losses to fish farms. Euglena sanguinea produces euglenophycin, a toxin similar in structure to solenopsin, an alkaloid found in fire ant venom. It was proved that euglenophycin exhibits not...

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Bibliographic Details
Published in:Journal of applied phycology 2018-06, Vol.30 (3), p.1759-1763
Main Authors: Kulczycka, Agata, Łukomska-Kowalczyk, Maja, Zakryś, Bożena, Milanowski, Rafał
Format: Article
Language:English
Subjects:
Anticancer properties
Antitumor activity
Aquaculture
Biomedical and Life Sciences
Blooms
Detection
DNA
Ecology
Environmental monitoring
Euglena
Euglena sanguinea
Farms
Fires
Fish
Fish culture
Fish farms
Freshwater
Freshwater & Marine Ecology
Herbicides
Identification
Inland water environment
Life Sciences
Methods
Monitoring
Nucleotide sequence
PCR
Plant Physiology
Plant Sciences
Quantitation
Species
Spectrometry
Venom
Water reservoirs
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Summary:Euglena sanguinea Ehrenberg is the only known species of euglenids which forms toxic blooms causing tangible losses to fish farms. Euglena sanguinea produces euglenophycin, a toxin similar in structure to solenopsin, an alkaloid found in fire ant venom. It was proved that euglenophycin exhibits not only ichthyotoxic but also herbicidal and anticancer activity. Recently, a specific mass spectrometric method of identification and quantitation of euglenophycin was developed to facilitate monitoring of that toxin in freshwater ponds. Despite the recent taxonomic verifications, proper identification of E. sanguinea is still difficult, especially for less experienced researchers. Herein, we describe a simple method based on nested PCR amplification of the nSSU rDNA fragments to identify a single E. sanguinea cell and its detection in a sample of water. The method will further facilitate monitoring of water reservoirs, especially estimating the risk of toxic blooms.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-017-1376-z