Changes of liver metabolites following hepatectomy with ischemia reperfusion towards liver regeneration

Background Metabolome analysis is one of the omics which investigates the final product of a central dogma. Changes of liver metabolites during liver regeneration following hepatectomy (Hx) continue to remain unclear. The aim of the present study was to investigate the changes of liver metabolites f...

Full description

Saved in:
Bibliographic Details
Published in:Annals of gastroenterological surgery 2018-05, Vol.2 (3), p.204-211
Main Authors: Saito, Yu, Morine, Yuji, Iwahashi, Shuichi, Ikemoto, Tetsuya, Imura, Satoru, Yamanaka‐Okumura, Hisami, Hirayama, Akiyoshi, Soga, Tomoyoshi, Tomita, Masaru, Shimada, Mitsuo
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Amino acids
Chromatography
Energy consumption
Ethics
Fatty acids
Hepatitis
Ischemia
ischemia reperfusion
Liver
liver regeneration
Mass spectrometry
Metabolism
Metabolites
metabolome analysis
Original
Principal components analysis
Sarcopenia
Scientific imaging
Serotonin
Software
Statistical analysis
Studies
tryptophan
Tumor necrosis factor-TNF
valine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Metabolome analysis is one of the omics which investigates the final product of a central dogma. Changes of liver metabolites during liver regeneration following hepatectomy (Hx) continue to remain unclear. The aim of the present study was to investigate the changes of liver metabolites following Hx with ischemia reperfusion (I/R) towards liver regeneration. Methods Twenty‐three patients who underwent Hx were enrolled in this study. Non‐tumor tissues were sampled immediately before and after Hx and a comparison was made between the liver samples taken before and after Hx using capillary electrophoresis (CE)—time‐of‐flight mass spectrometry (TOFMS) as metabolome analysis. Results The metabolic pathway showed that there was a significant increase in “lactate” following Hx. There was a significant decrease in metabolites only in the first half of the tricarboxylic acid cycle (TCA) cycle, and adenosine triphosphate (ATP) by anaerobiotic glycolysis did not occur in time for energy consumption of the Hx. Principal component analysis revealed remarkably different component profiles between the samples taken before and after Hx. One hundred and three metabolites were selected as critical metabolites for separating components. Valine and tryptophan increased significantly after Hx and they were regulated by resected liver volume, ischemic time and liver function. Conclusion The liver metabolites changed remarkably between before and after Hx. Especially, liver valine and tryptophan were increased. We investigated the changes of liver metabolites following hepatectomy (Hx) with ischemia reperfusion (I/R) towards liver regeneration using metabolome analysis.
ISSN:2475-0328
2475-0328
DOI:10.1002/ags3.12058