Difructose dianhydride improves intestinal calcium absorption, wound healing, and barrier function

Difructose dianhydride improves intestinal calcium absorption, wound healing, and barrier function

The gastrointestinal tract (GIT) is critical for nutrient absorption and is an important barrier against harmful pathogens and antigens. Difructose anhydrides (DFA)-IVs are nondigestible disaccharides that enhance calcium and iron absorption by affecting the intestinal epithelial tissue. However, th...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.7813-13, Article 7813
Main Authors: Lee, Sang In, Kim, In Ho
Format: Article
Language:eng
Subjects:
Antigens
Breast muscle
Calcium (blood)
Calcium absorption
Calcium permeability
Cell proliferation
Dextran
Digestibility
Disaccharides
Epithelial cells
Fluorescein isothiocyanate
Gastrointestinal tract
Intestine
Iron
Isothiocyanate
Lipopolysaccharides
Liver
Permeability
Small intestine
Wound healing
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Summary:The gastrointestinal tract (GIT) is critical for nutrient absorption and is an important barrier against harmful pathogens and antigens. Difructose anhydrides (DFA)-IVs are nondigestible disaccharides that enhance calcium and iron absorption by affecting the intestinal epithelial tissue. However, their effects on intestinal functions are not fully understood. In this study, we performed a feeding trial and found that dietary DFA-IVs improved growth performance, relative breast muscle and liver weight, and digestibility and blood calcium and iron concentrations in broilers. Additionally, dietary DFA-IVs increased expression of genes related to growth in the liver, muscle development, and absorption of calcium and iron in the intestine. In vitro experiments revealed that DFA-IV treatment improved intestinal wound-healing (migration, proliferation, and differentiation) after lipopolysaccharide (LPS) challenge in small intestinal epithelial cells. Furthermore, DFA-IV treatment enhanced the intestinal barrier function, which increased the transepithelial electrical resistance (TEER) and decreased the permeability of fluorescein isothiocyanate-dextran (FD-4) after LPS challenge in small intestinal epithelial cells. Collectively, these data indicate that DFA-IV could be used as a feed additive to enhance calcium and iron absorption by affecting the intestinal wound healing and permeability. This study may help improve our understanding of the molecular effects of DFA-IV on the intestine.
ISSN:2045-2322
2045-2322