A flexible MHC class I multimer loading system for large-scale detection of antigen-specific T cells

Adaptive immunity is initiated by T cell recognition of specific antigens presented by major histocompatibility complexes (MHCs). MHC multimer technology has been developed for the detection, isolation, and characterization of T cells in infection, autoimmunity, and cancer. Here, we present a simple...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of experimental medicine 2018-05, Vol.215 (5), p.1493-1504
Main Authors: Luimstra, Jolien J, Garstka, Malgorzata A, Roex, Marthe C J, Redeker, Anke, Janssen, George M C, van Veelen, Peter A, Arens, Ramon, Falkenburg, J H Frederik, Neefjes, Jacques, Ovaa, Huib
Format: Article
Language:English
Subjects:
Adaptive immunity
Antigens
Autoimmunity
Cancer
CD8 antigen
Cell recognition
Constitution
Cytomegalovirus
Epitopes
High temperature
Histocompatibility antigen H-2
Histocompatibility antigen HLA
Immunity
Low temperature
Lymphocytes
Lymphocytes T
Major histocompatibility complex
Monitoring
Peptides
Reagents
T cell receptors
Technology
Temperature
Temperature effects
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adaptive immunity is initiated by T cell recognition of specific antigens presented by major histocompatibility complexes (MHCs). MHC multimer technology has been developed for the detection, isolation, and characterization of T cells in infection, autoimmunity, and cancer. Here, we present a simple, fast, flexible, and efficient method to generate many different MHC class I (MHC I) multimers in parallel using temperature-mediated peptide exchange. We designed conditional peptides for HLA-A*02:01 and H-2K that form stable peptide-MHC I complexes at low temperatures, but dissociate when exposed to a defined elevated temperature. The resulting conditional MHC I complexes, either alone or prepared as ready-to-use multimers, can swiftly be loaded with peptides of choice without additional handling and within a short time frame. We demonstrate the ease and flexibility of this approach by monitoring the antiviral immune constitution in an allogeneic stem cell transplant recipient and by analyzing CD8 T cell responses to viral epitopes in mice infected with lymphocytic choriomeningitis virus or cytomegalovirus.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.20180156