MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity in Caenorhabditis elegans

Covalent attachment of ubiquitin to substrate proteins changes their function or marks them for proteolysis, and the specificity of ubiquitin attachment is mediated by the numerous E3 ligases encoded by animals. Mind Bomb is an essential E3 ligase during Notch pathway signaling in insects and verteb...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 2018-05, Vol.209 (1), p.173-193
Main Authors: Ratliff, Miriam, Hill-Harfe, Katherine L, Gleason, Elizabeth J, Ling, Huiping, Kroft, Tim L, L'Hernault, Steven W
Format: Article
Language:English
Subjects:
2-Methylisoborneol
Alleles
Animals
Caenorhabditis elegans
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
Coding
Drosophila
Gene expression
Gene Expression Regulation, Developmental
Genetics
Glucagon-Like Peptide 1 - metabolism
Hermaphrodites
Homology
Insects
Investigations
Kinases
LIN-12 protein
Loss of Function Mutation
Meiosis
Molecular chains
Mutants
Mutation
Nematodes
Notch protein
Phenotype
Phenotypes
Proteins
Proteolysis
Receptors, Notch - metabolism
Ribonucleic acid
RNA
RNA-mediated interference
Screens
Signal Transduction
Spermatids
Spermatocytes
Spermatogenesis
Spermatogenesis - genetics
Spermatozoa
Substrates
Tubulin
Ubiquitin
Ubiquitin-protein ligase
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Vertebrates
Viral infections
Vulva
Worms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Covalent attachment of ubiquitin to substrate proteins changes their function or marks them for proteolysis, and the specificity of ubiquitin attachment is mediated by the numerous E3 ligases encoded by animals. Mind Bomb is an essential E3 ligase during Notch pathway signaling in insects and vertebrates. While encodes a Mind Bomb homolog ( ), it has never been recovered in the extensive Notch suppressor/enhancer screens that have identified numerous pathway components. Here, we show that null mutants have a spermatogenesis-defective phenotype that results in a heterogeneous mixture of arrested spermatocytes, defective spermatids, and motility-impaired spermatozoa. mutants also have chromosome segregation defects during meiosis, molecular null mutants are intrinsically temperature-sensitive, and many spermatids contain large amounts of tubulin. These phenotypic features are similar to the endogenous RNA intereference (RNAi) mutants, but mutants do not affect RNAi. MIB-1 protein is expressed throughout the germ line with peak expression in spermatocytes followed by segregation into the residual body during spermatid formation. expression, while upregulated during spermatogenesis, also occurs somatically, including in vulva precursor cells. Here, we show that mutants suppress both and ( Notch) gain-of-function mutants, restoring anchor cell formation and a functional vulva to the former and partly restoring oocyte production to the latter. However, suppressed hermaphrodites are only observed when grown at 25°, and they are self-sterile. This probably explains why was not previously recovered as a Notch pathway component in suppressor/enhancer selection experiments.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.118.300807