A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells: Analysis of the Mechanism of Peritoneal Dissemination Using cDNA Macroarrays

We established a new cell line, NUGC‐3P4T, with high peritoneal metastatic disseminating potential in nude mice. NUGC‐3P4T cells were derived from the human gastric carcinoma line NUGC‐3, which has low capacity for peritoneal dissemination. NUGC‐3P4T cells developed peritoneal dissemination in 10/10...

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Bibliographic Details
Published in:Cancer science 2001-07, Vol.92 (7), p.748-754
Main Authors: Nomura, Hiroki, Nishimori, Hidefumi, Yasoshima, Takahiro, Hata, Fumitake, Sogahata, Katsuya, Tanaka, Hiroshi, Nakajima, Futoshi, Ikeda, Shinichiro, Kamiguchi, Kenjiro, Isomura, Hiroshi, Sato, Noriyuki, Denno, Ryuichi, Hirata, Koichi
Format: Article
Language:English
Subjects:
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
cDNA macroarray
Cell adhesion
Cell Adhesion - physiology
Cell Movement - physiology
Disease Models, Animal
Endothelial Growth Factors - biosynthesis
Endothelial Growth Factors - genetics
Experimental digestive system and abdominal tumors
Female
Gastric cancer
Gastric cancer lines
Gene Expression Regulation, Neoplastic
Humans
Immunomodulation
Interleukin-8 - biosynthesis
Interleukin-8 - genetics
Interleukins
Laminin
Lymphokines - biosynthesis
Lymphokines - genetics
Medical sciences
Metastases
Metastasis
Mice
Mice, Inbred BALB C
Mice, Nude
mRNA
Nude mice
Oligonucleotide Array Sequence Analysis
Peritoneal dissemination model
Peritoneal Neoplasms - genetics
Peritoneal Neoplasms - metabolism
Peritoneal Neoplasms - secondary
Peritoneum
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Tumor Cells, Cultured
Tumorigenicity
Tumors
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:We established a new cell line, NUGC‐3P4T, with high peritoneal metastatic disseminating potential in nude mice. NUGC‐3P4T cells were derived from the human gastric carcinoma line NUGC‐3, which has low capacity for peritoneal dissemination. NUGC‐3P4T cells developed peritoneal dissemination in 10/10 (100%) mice, whereas the parental NUGC‐3 cells developed dissemination in 1/5 (20.0%) mice. The metastatic foci in the peritoneum showed essentially the same histological appearance as those induced by parental cells. The tumorigenicity, the motile activity and the adhesive activity to the laminin of NUGC‐3P4T cells were stronger than those of NUGC‐3 cells. Production of IL‐8 was significantly higher in NUGC‐3P4T than in NUGC‐3. cDNA macroarrays analysis showed that a variety of cytokines, interleukins, and other immunomodulators and their receptors were up‐ or down‐regulated at the mRNA level in NUGC‐3P4T cells, compared with NUGC‐3 cells. Thus, this unique cell line and in vivo model might be useful to study the biology of peritoneal dissemination of human gastric cancer.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.2001.tb01157.x