Analyses of Oxidative DNA Damage and Its Repair Activity in the Livers of 3′‐Methyl‐4‐dimethylaminoazobenzene‐treated Rodents

We measured the levels of 8‐hydroxyguanine (8‐OH‐Gua) and its repair activity in the livers of the Donryu rat, the carcinogen‐resistant DRH rat, and the ddy mouse, which were fed a 0.06% 3′‐ methyl‐4‐dimethylaminoazobenzene (3′‐MeDAB)‐containing diet. In a short‐term rat experiment (maximum 2 months...

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Bibliographic Details
Published in:Cancer science 2000-07, Vol.91 (7), p.681-685
Main Authors: Hirano, Takeshi, Higashi, Ken, Sakai, Akinori, Tsurudome, Yosuke, Ootsuyama, Yuko, RieKido, Kasai, Hiroshi
Format: Article
Language:English
Subjects:
3'-Methyl-4-dimethylaminoazobenzene
3′‐MeDAB
8‐Hydroxyguanine
8‐Oxoguanine DNA glycosylase 1
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Carcinogenresistant DRH rat
Carcinogens
Carcinogens - toxicity
Chemical agents
Coloring Agents - toxicity
Deoxyribonucleic acid
Disease Susceptibility
DNA
DNA - drug effects
DNA - metabolism
DNA damage
DNA Damage - physiology
DNA repair
DNA Repair - physiology
DNA-Formamidopyrimidine Glycosylase
Guanine - analogs & derivatives
Guanine - metabolism
Liver
Liver - drug effects
Liver - metabolism
Liver Neoplasms, Experimental - chemically induced
Liver Neoplasms, Experimental - genetics
Liver Neoplasms, Experimental - metabolism
Male
Medical sciences
Methyldimethylaminoazobenzene - toxicity
Mice
N-Glycosyl Hydrolases - biosynthesis
N-Glycosyl Hydrolases - genetics
Oxidative stress
Oxidative Stress - physiology
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
RNA, Messenger - metabolism
Time Factors
Tumors
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Summary:We measured the levels of 8‐hydroxyguanine (8‐OH‐Gua) and its repair activity in the livers of the Donryu rat, the carcinogen‐resistant DRH rat, and the ddy mouse, which were fed a 0.06% 3′‐ methyl‐4‐dimethylaminoazobenzene (3′‐MeDAB)‐containing diet. In a short‐term rat experiment (maximum 2 months), 3′‐MeDAB did not increase the 8‐OH‐Gua levels in the livers of the two rat strains, although it significantly increased the repair activity in only the Donryu rat liver at 1 and 2 months. After long‐term 3′‐MeDAB administration to the ddy mouse (8 months), the levels of 8‐OH‐Gua and its repair activity were increased in the liver by 3.6‐fold and 1.6‐fold, respectively. These experiments suggest that 3′‐MeDAB increases 8‐OH‐Gua generation in rodent liver DNA and the 8‐OH‐Gua repair assay is a reliable marker of cellular oxidative stress induced by carcinogens.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.2000.tb00999.x