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Sequential T Cell Response Involved in Tumor Rejection of Sarcoma, Meth A, in Syngeneic Mice
We investigated the type of T cell response involved in Meth A tumor rejection in primary immune and hyperimmune syngeneic mice. It was found that a CD4+ T cell‐mediated delayed‐type hypersensitivity (DTH) response activating non‐specific killer cells such as macrophages, NK and LAK cells, without a...
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Published in: | Cancer science 1998-06, Vol.89 (6), p.657-665 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We investigated the type of T cell response involved in Meth A tumor rejection in primary immune and hyperimmune syngeneic mice. It was found that a CD4+ T cell‐mediated delayed‐type hypersensitivity (DTH) response activating non‐specific killer cells such as macrophages, NK and LAK cells, without a specific CD8+ cytotoxic T lymphocyte (CTL) response, was the major immune response leading to Meth A tumor rejection in primary immune mice. In contrast, the specific CD8+ CTL response was the major response leading to the tumor rejection, in addition to CD4+ T cell‐mediated DTH response, in hyperimmune mice. Analysis of CD4+ T cell clones established from primary immune and hyperimmune spleen cells indicated that a CD4+ T cell clone (C9) of primary immune mice (although only one clone was established) was of Th1 type, and induced cytotoxicity in accessory cells by classic DTH in vitro. Eight CD4+ T cell clones were established from hyperimmune spleen cells. Six out of the eight clones were of the Th2 type and two were Th0‐like. However, no Th1‐type CD4+ T cell clone was established from hyperimmune spleen cells. All of these CD4+ T cell clones, even the Th2‐type clones, were capable of inducing cytotoxicity in vitro in T cell‐depleted accessory cells, as in an in vitro DTH response. We postulate on the basis of these results that the T cell response leading to Meth A tumor rejection in vivo sequentially changed from a CD4+ T cell‐mediated classic DTH response to a CD8+ CTL response, in addition to a cellular response mediated probably by Th2‐type cells, during the process of repeated immunization. |
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ISSN: | 0910-5050 1347-9032 1349-7006 1876-4673 |
DOI: | 10.1111/j.1349-7006.1998.tb03268.x |