Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae...

Full description

Saved in:
Bibliographic Details
Published in:Cell transplantation 2018-02, Vol.27 (2), p.256-263
Main Authors: Wen, Tung-Chou, Li, Yuan-Sheng, Rajamani, Karthyayani, Harn, Horng-Jyh, Lin, Shinn-Zong, Chiou, Tzyy-Wen
Format: Article
Language:English
Subjects:
Cell proliferation
Cinnamic acid
Cinnamomum osmophloeum
Growth factors
Keratinocyte growth factor
Leaves
Liquid chromatography
mRNA
Original
Plant extracts
Polymerase chain reaction
Vascular endothelial growth factor
Vibrissae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023
cites cdi_FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023
container_end_page 263
container_issue 2
container_start_page 256
container_title Cell transplantation
container_volume 27
creator Wen, Tung-Chou
Li, Yuan-Sheng
Rajamani, Karthyayani
Harn, Horng-Jyh
Lin, Shinn-Zong
Chiou, Tzyy-Wen
description In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth–related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.
doi_str_mv 10.1177/0963689717741139
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5898689</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_0963689717741139</sage_id><sourcerecordid>2313724706</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023</originalsourceid><addsrcrecordid>eNp1UcFu1DAQtRCILoU7J2SJC5fA2I5j54JULUuLWAkkuFuTxOm6SuLFTgr9-85qS4FKnDzWe_Nm3hvGXgp4K4Qx76CuVGVrQ3UphKofsZXQWhfK1vIxWx3g4oCfsGc5XwGAUVI_ZSeSAGOFXbG86Xvfzjz2fB2mCcc4LiOPeYz73RA91Z9x8ruQkG899vzsx-Ljkvnm15zw0DfxDz6NOPCvuA_DgHztB_qkOITeJ5wDMXDq-AWGxM9T_DnvnrMnPQ7Zv7h7T9m3j5vv64ti--X80_psW7SlhLkgT6CV7VTTmNZYrepKdNboxqpeVHVTtqg6DUpI2ZTQ1aVuUEkAC9KAVKfs_VF1vzSj71o_0caD26cwYrpxEYP7F5nCzl3Ga6dtbSk0EnhzJ5Aiuc6zG0NuyR0FQhE4CbIEihtKor5-QL2KS5rInJNKKCNLAxWx4MhqU8w5-f5-GQHucE_38J7U8upvE_cNvw9IhOJIyHjp_0z9r-AtTCaneg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2313724706</pqid></control><display><type>article</type><title>Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth</title><source>Publicly Available Content Database</source><source>Sage Journals GOLD Open Access 2024</source><source>PubMed Central</source><creator>Wen, Tung-Chou ; Li, Yuan-Sheng ; Rajamani, Karthyayani ; Harn, Horng-Jyh ; Lin, Shinn-Zong ; Chiou, Tzyy-Wen</creator><creatorcontrib>Wen, Tung-Chou ; Li, Yuan-Sheng ; Rajamani, Karthyayani ; Harn, Horng-Jyh ; Lin, Shinn-Zong ; Chiou, Tzyy-Wen</creatorcontrib><description>In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth–related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.</description><identifier>ISSN: 0963-6897</identifier><identifier>EISSN: 1555-3892</identifier><identifier>DOI: 10.1177/0963689717741139</identifier><identifier>PMID: 29637818</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Cell proliferation ; Cinnamic acid ; Cinnamomum osmophloeum ; Growth factors ; Keratinocyte growth factor ; Leaves ; Liquid chromatography ; mRNA ; Original ; Plant extracts ; Polymerase chain reaction ; Vascular endothelial growth factor ; Vibrissae</subject><ispartof>Cell transplantation, 2018-02, Vol.27 (2), p.256-263</ispartof><rights>The Author(s) 2018</rights><rights>The Author(s) 2018. This work is licensed under the Creative Commons Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2018 2018 SAGE Publications Inc, unless otherwise noted. Manuscript content on this site is licensed under Creative Commons Licenses</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023</citedby><cites>FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898689/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2313724706?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,21994,25783,27886,27957,27958,37047,37048,44625,44980,45368,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29637818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Tung-Chou</creatorcontrib><creatorcontrib>Li, Yuan-Sheng</creatorcontrib><creatorcontrib>Rajamani, Karthyayani</creatorcontrib><creatorcontrib>Harn, Horng-Jyh</creatorcontrib><creatorcontrib>Lin, Shinn-Zong</creatorcontrib><creatorcontrib>Chiou, Tzyy-Wen</creatorcontrib><title>Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth</title><title>Cell transplantation</title><addtitle>Cell Transplant</addtitle><description>In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth–related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.</description><subject>Cell proliferation</subject><subject>Cinnamic acid</subject><subject>Cinnamomum osmophloeum</subject><subject>Growth factors</subject><subject>Keratinocyte growth factor</subject><subject>Leaves</subject><subject>Liquid chromatography</subject><subject>mRNA</subject><subject>Original</subject><subject>Plant extracts</subject><subject>Polymerase chain reaction</subject><subject>Vascular endothelial growth factor</subject><subject>Vibrissae</subject><issn>0963-6897</issn><issn>1555-3892</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>PIMPY</sourceid><recordid>eNp1UcFu1DAQtRCILoU7J2SJC5fA2I5j54JULUuLWAkkuFuTxOm6SuLFTgr9-85qS4FKnDzWe_Nm3hvGXgp4K4Qx76CuVGVrQ3UphKofsZXQWhfK1vIxWx3g4oCfsGc5XwGAUVI_ZSeSAGOFXbG86Xvfzjz2fB2mCcc4LiOPeYz73RA91Z9x8ruQkG899vzsx-Ljkvnm15zw0DfxDz6NOPCvuA_DgHztB_qkOITeJ5wDMXDq-AWGxM9T_DnvnrMnPQ7Zv7h7T9m3j5vv64ti--X80_psW7SlhLkgT6CV7VTTmNZYrepKdNboxqpeVHVTtqg6DUpI2ZTQ1aVuUEkAC9KAVKfs_VF1vzSj71o_0caD26cwYrpxEYP7F5nCzl3Ga6dtbSk0EnhzJ5Aiuc6zG0NuyR0FQhE4CbIEihtKor5-QL2KS5rInJNKKCNLAxWx4MhqU8w5-f5-GQHucE_38J7U8upvE_cNvw9IhOJIyHjp_0z9r-AtTCaneg</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Wen, Tung-Chou</creator><creator>Li, Yuan-Sheng</creator><creator>Rajamani, Karthyayani</creator><creator>Harn, Horng-Jyh</creator><creator>Lin, Shinn-Zong</creator><creator>Chiou, Tzyy-Wen</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>AFRWT</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201802</creationdate><title>Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth</title><author>Wen, Tung-Chou ; Li, Yuan-Sheng ; Rajamani, Karthyayani ; Harn, Horng-Jyh ; Lin, Shinn-Zong ; Chiou, Tzyy-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cell proliferation</topic><topic>Cinnamic acid</topic><topic>Cinnamomum osmophloeum</topic><topic>Growth factors</topic><topic>Keratinocyte growth factor</topic><topic>Leaves</topic><topic>Liquid chromatography</topic><topic>mRNA</topic><topic>Original</topic><topic>Plant extracts</topic><topic>Polymerase chain reaction</topic><topic>Vascular endothelial growth factor</topic><topic>Vibrissae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wen, Tung-Chou</creatorcontrib><creatorcontrib>Li, Yuan-Sheng</creatorcontrib><creatorcontrib>Rajamani, Karthyayani</creatorcontrib><creatorcontrib>Harn, Horng-Jyh</creatorcontrib><creatorcontrib>Lin, Shinn-Zong</creatorcontrib><creatorcontrib>Chiou, Tzyy-Wen</creatorcontrib><collection>Sage Journals GOLD Open Access 2024</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health &amp; Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Tung-Chou</au><au>Li, Yuan-Sheng</au><au>Rajamani, Karthyayani</au><au>Harn, Horng-Jyh</au><au>Lin, Shinn-Zong</au><au>Chiou, Tzyy-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth</atitle><jtitle>Cell transplantation</jtitle><addtitle>Cell Transplant</addtitle><date>2018-02</date><risdate>2018</risdate><volume>27</volume><issue>2</issue><spage>256</spage><epage>263</epage><pages>256-263</pages><issn>0963-6897</issn><eissn>1555-3892</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth–related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>29637818</pmid><doi>10.1177/0963689717741139</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0963-6897
ispartof Cell transplantation, 2018-02, Vol.27 (2), p.256-263
issn 0963-6897
1555-3892
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5898689
source Publicly Available Content Database; Sage Journals GOLD Open Access 2024; PubMed Central
subjects Cell proliferation
Cinnamic acid
Cinnamomum osmophloeum
Growth factors
Keratinocyte growth factor
Leaves
Liquid chromatography
mRNA
Original
Plant extracts
Polymerase chain reaction
Vascular endothelial growth factor
Vibrissae
title Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T12%3A42%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Cinnamomum%20osmophloeum%20Kanehira%20Leaf%20Aqueous%20Extract%20on%20Dermal%20Papilla%20Cell%20Proliferation%20and%20Hair%20Growth&rft.jtitle=Cell%20transplantation&rft.au=Wen,%20Tung-Chou&rft.date=2018-02&rft.volume=27&rft.issue=2&rft.spage=256&rft.epage=263&rft.pages=256-263&rft.issn=0963-6897&rft.eissn=1555-3892&rft_id=info:doi/10.1177/0963689717741139&rft_dat=%3Cproquest_pubme%3E2313724706%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c420t-7740538d3bb7c7853961d875b83f169b4ca3d503122b40d945ba32008027023%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2313724706&rft_id=info:pmid/29637818&rft_sage_id=10.1177_0963689717741139&rfr_iscdi=true