Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae...

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Bibliographic Details
Published in:Cell transplantation 2018-02, Vol.27 (2), p.256-263
Main Authors: Wen, Tung-Chou, Li, Yuan-Sheng, Rajamani, Karthyayani, Harn, Horng-Jyh, Lin, Shinn-Zong, Chiou, Tzyy-Wen
Format: Article
Language:English
Subjects:
Cell proliferation
Cinnamic acid
Cinnamomum osmophloeum
Growth factors
Keratinocyte growth factor
Leaves
Liquid chromatography
mRNA
Original
Plant extracts
Polymerase chain reaction
Vascular endothelial growth factor
Vibrissae
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Summary:In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth–related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.
ISSN:0963-6897
1555-3892
DOI:10.1177/0963689717741139