The consequences of niche and physiological differentiation of archaeal and bacterial ammonia oxidisers for nitrous oxide emissions

High and low rates of ammonium supply are believed to favour ammonia-oxidising bacteria (AOB) and archaea (AOA), respectively. Although their contrasting affinities for ammonium are suggested to account for these differences, the influence of ammonia concentration on AOA and AOB has not been tested...

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Bibliographic Details
Published in:	The ISME Journal 2018-04, Vol.12 (4), p.1084-1093
Main Authors:	Hink, Linda, Gubry-Rangin, Cécile, Nicol, Graeme W, Prosser, James I
Format:	Article
Language:	eng
Subjects:	Ammonia Ammonia - metabolism Ammonium Archaea Archaea - metabolism Emissions Environmental conditions Environmental Sciences Environmental testing Fertilizers Microcosms Mineralization Nitrification Nitrogen dioxide Nitrous oxide Nitrous Oxide - analysis Nitrous Oxide - metabolism Organic fertilizers Organic matter Oxidation Oxidation-Reduction Sediment yield Soil - chemistry Soil Microbiology Urea Urea - metabolism
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Summary:	High and low rates of ammonium supply are believed to favour ammonia-oxidising bacteria (AOB) and archaea (AOA), respectively. Although their contrasting affinities for ammonium are suggested to account for these differences, the influence of ammonia concentration on AOA and AOB has not been tested under environmental conditions. In addition, while both AOB and AOA contribute to nitrous oxide (N O) emissions from soil, N O yields (N O-N produced per NO -N generated from ammonia oxidation) of AOA are lower, suggesting lower emissions when AOA dominate ammonia oxidation. This study tested the hypothesis that ammonium supplied continuously at low rates is preferentially oxidised by AOA, with lower N O yield than expected for AOB-dominated processes. Soil microcosms were supplied with water, urea or a slow release, urea-based fertiliser and 1-octyne (inhibiting only AOB) was applied to distinguish AOA and AOB activity and associated N O production. Low ammonium supply, from mineralisation of organic matter, or of the fertiliser, led to growth, ammonia oxidation and N O production by AOA only, with low N O yield. High ammonium supply, from free urea within the fertiliser or after urea addition, led to growth of both groups, but AOB-dominated ammonia oxidation was associated with twofold greater N O yield than that dominated by AOA. This study therefore demonstrates growth of both AOA and AOB at high ammonium concentration, confirms AOA dominance during low ammonium supply and suggests that slow release or organic fertilisers potentially mitigate N O emissions through differences in niche specialisation and N O production mechanisms in AOA and AOB.
ISSN:	1751-7362 1751-7370