Analytical Method Development and Validation for the Simultaneous Estimation of Abacavir and Lamivudine by Reversed-phase High-performance Liquid Chromatography in Bulk and Tablet Dosage Forms

Objective: A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Abacavir (ABAC) and Lamivudine (LAMI) in bulk and tablet dosage forms. Methods: The quantification was carried out using...

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Bibliographic Details
Published in:Pharmacognosy research 2018-01, Vol.10 (1), p.92-97
Main Authors: Raees Ahmad, Sufiyan, Patil, Lalit, Mohammed Usman, Mohammed, Imran, Mohammad, Akhtar, Rashid
Format: Article
Language:English
Subjects:
Abacavir
Acquired immune deficiency syndrome
AIDS
Analysis
Antiretroviral drugs
High performance liquid chromatography
HIV
Human immunodeficiency virus
Lamivudine
Methanol
Methods
Original
Pharmaceuticals
Phosphoric acid
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Summary:Objective: A simple rapid, accurate, precise, and reproducible validated reverse phase high performance liquid chromatography (HPLC) method was developed for the determination of Abacavir (ABAC) and Lamivudine (LAMI) in bulk and tablet dosage forms. Methods: The quantification was carried out using Symmetry Premsil C18 (250 mm × 4.6 mm, 5 μm) column run in isocratic way using mobile phase comprising methanol: water (0.05% orthophosphoric acid with pH 3) 83:17 v/v and a detection wavelength of 245 nm and injection volume of 20 μl, with a flow rate of 1 ml/min. Results: In the developed method, the retention times of ABAC and LAMI were found to be 3.5 min and 7.4 min, respectively. The method was validated in terms of linearity, precision, accuracy, limits of detection, limits of quantitation, and robustness in accordance with the International Conference on Harmonization guidelines. Conclusion: The assay of the proposed method was found to be 99% - 101%. The recovery studies were also carried out and mean % recovery was found to be 99% - 101%. The % relative standard deviation from reproducibility was found to be
ISSN:0974-8490
0976-4836
0974-8490
DOI:10.4103/pr.pr_96_17