Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1

VP22 is a major tegument protein of equine herpesvirus type 1 (EHV-1). In the present study, we examined functions of VP22 in EHV-1 replication by viral protein expression analyses in cells infected with the VP22-deficient virus. The expressions of several viral proteins in the cells infected with t...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2018, Vol.80(2), pp.311-315
Main Authors: OKADA, Ayaka, SUGANUMA, Shota, BADR, Yassien, OMATSU, Tsutomu, MIZUTANI, Tetsuya, OHYA, Kenji, FUKUSHI, Hideto
Format: Article
Language:English
Subjects:
Clonal deletion
EHV-1
Gene expression
ICP4
Post-transcription
Proteins
Tegument
Virology
Viruses
VP22
VP22 protein
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Summary:VP22 is a major tegument protein of equine herpesvirus type 1 (EHV-1). In the present study, we examined functions of VP22 in EHV-1 replication by viral protein expression analyses in cells infected with the VP22-deficient virus. The expressions of several viral proteins in the cells infected with the VP22-deficient virus were lower than those in the cells infected with the parent virus. One of the weakly expressed proteins was identified as ICP4, which is a major regulatory protein encoded by an immediate early gene of EHV-1. A real-time PCR analysis showed that the mRNA expression of ICP4 was the same in cells infected with the parent and VP22-deficient viruses. Hence, VP22 appears to promote synthesis of ICP4 post-transcriptionally.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.17-0380