Adult mouse eIF2Bε Arg191His astrocytes display a normal integrated stress response in vitro

Vanishing white matter (VWM) is a genetic childhood white matter disorder, characterized by chronic as well as episodic, stress provoked, neurological deterioration. Treatment is unavailable and patients often die within a few years after onset. VWM is caused by recessive mutations in the eukaryotic...

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Published in:Scientific reports 2018-02, Vol.8 (1), p.3773-10, Article 3773
Main Authors: Wisse, Lisanne E, Ter Braak, Timo J, van de Beek, Malu-Clair, van Berkel, Carola G M, Wortel, Joke, Heine, Vivi M, Proud, Chris G, van der Knaap, Marjo S, Abbink, Truus E M
Format: Article
Language:English
Subjects:
Astrocytes
Children
Initiation factor eIF-2
Mutation
Protein biosynthesis
Protein synthesis
Stress response
Substantia alba
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Summary:Vanishing white matter (VWM) is a genetic childhood white matter disorder, characterized by chronic as well as episodic, stress provoked, neurological deterioration. Treatment is unavailable and patients often die within a few years after onset. VWM is caused by recessive mutations in the eukaryotic initiation factor 2B (eIF2B). eIF2B regulates protein synthesis rates in every cell of the body. In normal cells, various types of cellular stress inhibit eIF2B activity and induce the integrated stress response (ISR). We have developed a VWM mouse model homozygous for the pathogenic Arg191His mutation in eIF2Bε (2b5 ), representative of the human disease. Neuropathological examination of VWM patient and mouse brain tissue suggests that astrocytes are primarily affected. We hypothesized that VWM astrocytes are selectively hypersensitive to ISR induction, resulting in a heightened response. We cultured astrocytes from wildtype and VWM mice and investigated the ISR in assays that measure transcriptional induction of stress genes, protein synthesis rates and cell viability. We investigated the effects of short- and long-term stress as well as stress recovery. We detected congruent results amongst the various assays and did not detect a hyperactive ISR in VWM mouse astrocytes.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-21885-x