A Single Mechanosensitive Channel Protects Francisella tularensis subsp. holarctica from Hypoosmotic Shock and Promotes Survival in the Aquatic Environment

subsp. is found in North America and much of Europe and causes the disease tularemia in humans and animals. An aquatic cycle has been described for this subspecies, which has caused waterborne outbreaks of tularemia in at least 10 countries. In this study, we sought to identify the mechanosensitive...

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Bibliographic Details
Published in:Applied and environmental microbiology 2018-03, Vol.84 (5)
Main Authors: Williamson, David R, Dewan, Kalyan K, Patel, Tanmay, Wastella, Catherine M, Ning, Gang, Kirimanjeswara, Girish S
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antibiotics
Aquatic animals
Aquatic environment
Bacteria
Cell morphology
Cell size
Clonal deletion
Cytology
Cytoplasm
Detergents
Disease transmission
Francisella tularensis
Francisella tularensis - physiology
Fresh Water
Hydrogen peroxide
Mammals
Mechanotransduction, Cellular - physiology
Mice
Mice, Inbred C57BL
Microbial Ecology
Osmosis
Outbreaks
Proteins
Salt Stress
Specific Pathogen-Free Organisms
Survival
Tularemia
Virulence
Waterborne diseases
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Summary:subsp. is found in North America and much of Europe and causes the disease tularemia in humans and animals. An aquatic cycle has been described for this subspecies, which has caused waterborne outbreaks of tularemia in at least 10 countries. In this study, we sought to identify the mechanosensitive channel(s) required for the bacterium to survive the transition from mammalian hosts to freshwater, which is likely essential for the transmission of the bacterium between susceptible hosts. A single 165-amino-acid MscS-type mechanosensitive channel ( MscS) was found to protect subsp. from hypoosmotic shock, despite lacking much of the cytoplasmic vestibule domain found in well-characterized MscS proteins from other organisms. The deletion of this channel did not affect virulence within the mammalian host; however, MscS was required to survive the transition from the host niche to freshwater. The deletion of MscS did not alter the sensitivity of subsp. to detergents, H O , or antibiotics, suggesting that the role of MscS is specific to protection from hypoosmotic shock. The deletion of MscS also led to a reduced average cell size without altering gross cell morphology. The mechanosensitive channel identified and characterized in this study likely contributes to the transmission of tularemia between hosts by allowing the bacterium to survive the transition from mammalian hosts to freshwater. The contamination of freshwater by subsp. has resulted in a number of outbreaks of tularemia. Invariably, the contamination originates from the carcasses or excreta of infected animals and thus involves an abrupt osmotic downshock as the bacteria enter freshwater. How survives this drastic change in osmolarity has not been clear, but here we report that a single mechanosensitive channel protects the bacterium from osmotic downshock. This channel is functional despite lacking much of the cytoplasmic vestibule domain that is present in better-studied organisms such as ; this report builds on previous studies that have suggested that parts of this domain are dispensable for downshock protection. These findings extend our understanding of the aquatic cycle and ecological persistence of , with further implications for mechanosensitive channel biology.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.02203-17