Cytosolic proteome profiling of monocytes for male osteoporosis

Summary In male Caucasians with discordant hip bone mineral density (BMD), we applied the subcellular separation and proteome profiling to investigate the monocytic cytosol. Three BMD-associated proteins (ALDOA, MYH14, and Rap1B) were identified based on multiple omics evidence, and they may influen...

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Published in:	Osteoporosis international 2017-03, Vol.28 (3), p.1035-1046
Main Authors:	Zhu, W., Shen, H., Zhang, J-G., Zhang, L., Zeng, Y., Huang, H-L., Zhao, Y-C., He, H., Zhou, Y., Wu, K-H., Tian, Q., Zhao, L-J., Deng, F-Y., Deng, H-W.
Format:	Article
Language:	eng
Subjects:	Absorptiometry, Photon Adult Aging Bioinformatics Bone Density - genetics Bone Density - physiology Bone mineral density Bone turnover Cytokines Cytosol Cytosol - metabolism Endocrinology Fructose-Bisphosphate Aldolase - biosynthesis Fructose-Bisphosphate Aldolase - genetics Gene Expression Profiling - methods Gene Expression Regulation - physiology Gene Ontology Gene Regulatory Networks - physiology Hip Humans Male Medicine Medicine & Public Health Middle Aged Monocytes Monocytes - metabolism Myosin Heavy Chains - biosynthesis Myosin Heavy Chains - genetics Myosin Type II - biosynthesis Myosin Type II - genetics Original Article Orthopedics Osteoclasts Osteoporosis Osteoporosis - blood Osteoporosis - genetics Osteoporosis - physiopathology Osteoprogenitor cells Peripheral blood Proteins Proteome - genetics Proteome - metabolism Proteomics Proteomics - methods Public health rap GTP-Binding Proteins - biosynthesis rap GTP-Binding Proteins - genetics Rheumatology
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Summary:	Summary In male Caucasians with discordant hip bone mineral density (BMD), we applied the subcellular separation and proteome profiling to investigate the monocytic cytosol. Three BMD-associated proteins (ALDOA, MYH14, and Rap1B) were identified based on multiple omics evidence, and they may influence the pathogenic mechanisms of osteoporosis by regulating the activities of monocytes. Introduction Osteoporosis is a serious public health problem, leading to significant mortality not only in aging females but also in males. Peripheral blood monocytes (PBMs) play important roles in bone metabolism by acting as precursors of osteoclasts and producing cytokines important for osteoclast development. The first cytosolic sub-proteome profiling analysis was performed in male PBMs to identify differentially expressed proteins (DEPs) that are associated with BMDs and risk of osteoporosis. Methods Here, we conducted a comparative proteomics analysis in PBMs from Caucasian male subjects with discordant hip BMD (29 low BMD vs. 30 high BMD). To decrease the proteome complexity and expand the coverage range of the cellular proteome, we separated the PBM proteome into several subcellular compartments and focused on the cytosolic fractions, which are involved in a wide range of fundamental biochemical processes. Results Of the total of 3796 detected cytosolic proteins, we identified 16 significant ( P
ISSN:	0937-941X 1433-2965