Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline

Metal uptake is vital for all living organisms. In metal scarce conditions a common bacterial strategy consists in the biosynthesis of metallophores, their export in the extracellular medium and the recovery of a metal-metallophore complex through dedicated membrane transporters. Staphylopine is a r...

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Published in:Scientific reports 2017-12, Vol.7 (1), p.17132-10, Article 17132
Main Authors: Lhospice, Sébastien, Gomez, Nicolas Oswaldo, Ouerdane, Laurent, Brutesco, Catherine, Ghssein, Ghassan, Hajjar, Christine, Liratni, Ahmed, Wang, Shuanglong, Richaud, Pierre, Bleves, Sophie, Ball, Geneviève, Borezée-Durant, Elise, Lobinski, Ryszard, Pignol, David, Arnoux, Pascal, Voulhoux, Romé
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biosynthesis
Chelating Agents - metabolism
Chemical Sciences
Histidine
Ketoglutaric acid
Mimicry
Nickel
Nicotianamine
Oligopeptides - metabolism
Operon
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - metabolism
Pseudomonas Infections - microbiology
Pyruvic acid
Stereochemistry
Zinc
Zinc - metabolism
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Summary:Metal uptake is vital for all living organisms. In metal scarce conditions a common bacterial strategy consists in the biosynthesis of metallophores, their export in the extracellular medium and the recovery of a metal-metallophore complex through dedicated membrane transporters. Staphylopine is a recently described metallophore distantly related to plant nicotianamine that contributes to the broad-spectrum metal uptake capabilities of Staphylococcus aureus. Here we characterize a four-gene operon (PA4837-PA4834) in Pseudomonas aeruginosa involved in the biosynthesis and trafficking of a staphylopine-like metallophore named pseudopaline. Pseudopaline differs from staphylopine with regard to the stereochemistry of its histidine moiety associated with an alpha ketoglutarate moiety instead of pyruvate. In vivo, the pseudopaline operon is regulated by zinc through the Zur repressor. The pseudopaline system is involved in nickel uptake in poor media, and, most importantly, in zinc uptake in metal scarce conditions mimicking a chelating environment, thus reconciling the regulation of the cnt operon by zinc with its function as the main zinc importer under these metal scarce conditions.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-16765-9