Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets

Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopath...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-11, Vol.7 (1), p.16555-11, Article 16555
Main Authors: Oliveira, Thalita E S, Michelazzo, Mariana M Z, Fernandes, Thiago, de Oliveira, Admilton G, Leme, Raquel A, Alfieri, Alice F, Alfieri, Amauri A, Headley, Selwyn A
Format: Article
Language:English
Subjects:
Ballooning
Capillaries
Choroid plexus
Choroiditis
Circulatory system
Endothelial cells
Endothelium
Enteric nervous system
Enteritis
Enterocytes
Epithelium
Gangrene
Immunoreactivity
Inflammation
Meningoencephalitis
Necrosis
Neonates
Neurodegeneration
Polymerase chain reaction
Small intestine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopathology revealed ballooning degeneration of transitional epithelium, nonsuppurative meningoencephalitis, plexus choroiditis, and atrophic enteritis. RT-PCR identified SVA in all tissues evaluated and sequencing confirmed these results. Positive immunoreactivity to SVA was observed in endothelial and epithelial tissues of all organs evaluated. Semithin analysis revealed vacuolization of apical enterocytes of the small intestine, balloon degeneration and necrosis of endothelial cells of the choroid plexus (CP) and nonsuppurative choroid plexitis. Ultrathin evaluation demonstrated hydropic degeneration of apical enterocytes, degeneration and necrosis of endothelium of CP fenestrated capillaries, degeneration of ependymocytes associated with intralesional viral particles. It is proposed that SVA initially infects apical enterocytes of newborn piglets and probably enters the circulatory system with entry to the brain via the CP, by first producing an initial inflammatory reaction, with subsequent encephalitic dissemination. Consequently, SVA probably uses an enteric-neurological method of dissemination.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-16407-0