Candida albicans/Staphylococcus aureus Dual-Species Biofilm as a Target for the Combination of Essential Oils and Fluconazole or Mupirocin

The effectiveness of essential oils (EOs), fluconazole (FLU) and mupirocin (MUP) used alone or in combination against mono-species and mixed Candida albicans/Staphylococcus aureus biofilms was examined. An experimentally established dual-species biofilm model, verified by fluorescence microscopy and...

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Bibliographic Details
Published in:Mycopathologia (1975) 2017-12, Vol.182 (11-12), p.989-995
Main Authors: Budzyńska, Aleksandra, Różalska, Sylwia, Sadowska, Beata, Różalska, Barbara
Format: Article
Language:English
Subjects:
Analysis
Antibacterial agents
Antibiotics
Antifungal agents
Antifungal Agents - pharmacology
Biofilms
Biofilms - drug effects
Biomedical and Life Sciences
Candida albicans
Candida albicans - drug effects
Clove Oil - pharmacology
Drug Combinations
Essential oils
Eukaryotic Microbiology
Fluconazole
Fluconazole - pharmacology
Fluorescence
Fluorescence microscopy
Geranium - chemistry
Hyphae - growth & development
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Minimum inhibitory concentration
Mupirocin
Mupirocin - pharmacology
Oils & fats
Oils, Volatile - pharmacology
Plant Oils - pharmacology
Plant Sciences
Species
Staphylococcus aureus
Staphylococcus aureus - drug effects
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Summary:The effectiveness of essential oils (EOs), fluconazole (FLU) and mupirocin (MUP) used alone or in combination against mono-species and mixed Candida albicans/Staphylococcus aureus biofilms was examined. An experimentally established dual-species biofilm model, verified by fluorescence microscopy and viable cell counting, was used. Selected commercial EOs were tested: geranium, citronella and clove oils, which have been chemically characterized and found to differ in the content of the main components (qualitative and quantitative). As expected, C. albicans and S. aureus biofilms were less susceptible to fluconazole and mupirocin action, respectively, compared to the planktonic counterparts. However, the drug effectiveness in combination with the EOs was significantly improved, giving enhancement of biofilm eradication than caused by the antibiotics alone. Moreover, dual-species biofilm formation was limited by sub-MIC of EOs, and preformed mixed biofilm was eliminated more efficiently by combined action of drugs and EOs.
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-017-0192-y