Planarian Epidermal Stem Cells Respond to Positional Cues to Promote Cell-Type Diversity

Successful regeneration requires that progenitors of different lineages form the appropriate missing cell types. However, simply generating lineages is not enough. Cells produced by a particular lineage often have distinct functions depending on their position within the organism. How this occurs in...

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Bibliographic Details
Published in:Developmental cell 2017-03, Vol.40 (5), p.491-504.e5
Main Authors: Wurtzel, Omri, Oderberg, Isaac M., Reddien, Peter W.
Format: Article
Language:English
Subjects:
Animals
Biomarkers - metabolism
BMP signaling
Body Patterning - genetics
Bone Morphogenetic Proteins - metabolism
Cell Differentiation - genetics
Cell Lineage
Epidermal Cells
epidermis
Epidermis - metabolism
Gene Expression Regulation, Developmental
Mitosis - genetics
Models, Biological
neoblast
patterning
planaria
Planarians - cytology
progenitors
regeneration
RNA Interference
Sequence Analysis, RNA
Signal Transduction - genetics
Single-Cell Analysis
single-cell sequencing
stem cells
Stem Cells - cytology
Stem Cells - metabolism
Transcription Factors - metabolism
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Summary:Successful regeneration requires that progenitors of different lineages form the appropriate missing cell types. However, simply generating lineages is not enough. Cells produced by a particular lineage often have distinct functions depending on their position within the organism. How this occurs in regeneration is largely unexplored. In planarian regeneration, new cells arise from a proliferative cell population (neoblasts). We used the planarian epidermal lineage to study how the location of adult progenitor cells results in their acquisition of distinct functional identities. Single-cell RNA sequencing of epidermal progenitors revealed the emergence of distinct spatial identities as early in the lineage as the epidermal neoblasts, with further pre-patterning occurring in their post-mitotic migratory progeny. Establishment of dorsal-ventral epidermal identities and functions, in response to BMP signaling, required neoblasts. Our work identified positional signals that activate regionalized transcriptional programs in the stem cell population and subsequently promote cell-type diversity in the epidermis. [Display omitted] •Single-cell RNA sequencing reveals spatial patterning in a progenitor pool•Planarian epidermal neoblasts read their position in the animal•Position-specific transcriptional programs in progenitors generate cell-type diversity•Patterning signals from muscle regulate epidermal neoblast positional identity Wurtzel et al. examine how in planarian regeneration, adult progenitor cell location contributes to acquisition of distinct functional identities. They provide insight for how progenitors in the epidermis read their position in the animal to activating region-specific transcription, which is ultimately propagated to differentiated progeny to generate the required cellular functions.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2017.02.008