Molecular characterization of emaraviruses associated with Pigeonpea sterility mosaic disease

Sterility Mosaic Disease (SMD) of pigeonpea (Cajanus cajan (L.) Millspaugh) is a complex disease due to various factors including the presence of a mixed infection. Comparison of dsRNA profile and small RNA (sRNA) deep sequencing analysis of samples from three locations revealed the presence of Pige...

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Bibliographic Details
Published in:Scientific reports 2017-09, Vol.7 (1), p.11831-20, Article 11831
Main Authors: Kumar, Surender, Subbarao, B L, Hallan, Vipin
Format: Article
Language:English
Subjects:
Binding sites
Cajanus - genetics
Cajanus - metabolism
Cajanus - virology
Cajanus cajan
Double-stranded RNA
Endonuclease
Enzyme-linked immunosorbent assay
Genomes
High-Throughput Nucleotide Sequencing
Homology
Inoculation
Mixed infection
Mosaic disease
Movement protein
Nucleocapsids
Nucleotide sequence
Plant Diseases - genetics
Plant Diseases - virology
Plant virus diseases
Plant Viruses - genetics
Plant Viruses - metabolism
Protein Domains
Ribonucleic acid
RNA
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
RNA, Viral - genetics
RNA, Viral - metabolism
Sterility
Transcription
Viral Proteins - genetics
Viral Proteins - metabolism
Viruses
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Summary:Sterility Mosaic Disease (SMD) of pigeonpea (Cajanus cajan (L.) Millspaugh) is a complex disease due to various factors including the presence of a mixed infection. Comparison of dsRNA profile and small RNA (sRNA) deep sequencing analysis of samples from three locations revealed the presence of Pigeonpea sterility mosaic virus-I and II (PPSMV-I and II) from Chevella and only PPSMV-II from Bengaluru and Coimbatore. PPSMV-I genome consisted of four while PPSMV-II encompassed six RNAs. The two viruses have modest sequence homology between their corresponding RNA 1-4 encoding RdRp, glycoprotein precursor, nucleocapsid and movement proteins and the corresponding orthologs of other emaraviruses. However, PPSMV-II is more related to Fig mosaic virus (FMV) than to PPSMV-I. ELISA based detection methodology was standardized to identify these two viruses, uniquely. Mite inoculation of sub-isolate Chevella sometimes resulted in few- to- many pigeonpea plants containing PPSMV-I alone. The study shows that (i) the N-terminal region of RdRp (SRD-1) of both the viruses contain "cap-snatching" endonuclease domain and a 13 AA cap binding site at the C-terminal, essential for viral cap-dependent transcription similar to the members of Bunyaviridae family and (ii) P4 is the movement protein and may belong to '30 K superfamily' of MPs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-11958-8