limited host range of an Agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range T-region

The host range of Agrobacterium tumefaciens strain LBA649 (pTiAg57) is limited to grapevine and a few other plant species. Its host range was extended through the introduction of the T‐region from the wide host range octopine plasmid pTiAch5. In contrast, R prime plasmids harboring the entire wide h...

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Bibliographic Details
Published in:The EMBO journal 1984-12, Vol.3 (13), p.3043-3047
Main Authors: Hoekema, A, Pater, B.S. de, Fellinger, A.J, Hooykaas, P.J.J, Schilperoort, R.A
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Applied sciences
binary vector system
crown gall
cytokinins
cytokinin‐gene
Exact sciences and technology
genes
host range
Other techniques and industries
plasmids
transformation
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Summary:The host range of Agrobacterium tumefaciens strain LBA649 (pTiAg57) is limited to grapevine and a few other plant species. Its host range was extended through the introduction of the T‐region from the wide host range octopine plasmid pTiAch5. In contrast, R prime plasmids harboring the entire wide host range virulence region were unable to achieve this effect. Via site‐directed mutagenesis a search was performed to identify the T‐DNA genes which were responsible for the observed host range extension. Inactivation of one of the onc‐genes (the cyt gene) was found to abolish the capacity of the T‐region to extend the host range of LBA649. Therefore, we cloned the cyt gene into a disarmed T‐region plant vector and used it in complementation studies with pTiAg57 via the binary vector strategy. We show that the mere presence of the cyt gene from a wide host range Ti plasmid is sufficient to extend the host range of LBA649 to certain plants. We conclude that the limited host range of LBA649 is not caused by a lack of recognition of plants but is mainly due to the absence or inactivity of a cyt gene in the T‐region of pTiAg57.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1984.tb02255.x