Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells

Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scan...

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Bibliographic Details
Published in:Nucleic acids research 2017-07, Vol.45 (13), p.7666-7680
Main Authors: Ilyin, Artem A, Ryazansky, Sergei S, Doronin, Semen A, Olenkina, Oxana M, Mikhaleva, Elena A, Yakushev, Evgeny Y, Abramov, Yuri A, Belyakin, Stepan N, Ivankin, Anton V, Pindyurin, Alexey V, Gvozdev, Vladimir A, Klenov, Mikhail S, Shevelyov, Yuri Y
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Argonaute Proteins - chemistry
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Chromatin - genetics
Chromatin - metabolism
DNA Transposable Elements
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Gene regulation, Chromatin and Epigenetics
Gene Silencing
Genome, Insect
Genomic Instability
Models, Biological
Nuclear Pore - genetics
Nuclear Pore - metabolism
Ovary - cytology
Ovary - metabolism
Protein Binding
Protein Interaction Domains and Motifs
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Here we report the DamID-seq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the protein-coding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkx355