Metabolomic profiling for identification of metabolites and relevant pathways for taurine in hepatic stellate cells

AIM To develop a reliable and simple method to identify important biological metabolites and relevant pathways for taurine in hepatic stellate cells(HSCs), in order to provide more data for taurine therapy.METHODS All the biological samples were analyzed by using highperformance liquid chromatograph...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2017-08, Vol.23 (31), p.5713-5721
Main Authors: Deng, Xin, Liang, Xing-Qiu, Lu, Fei-Guo, Zhao, Xiao-Fang, Fu, Lei, Liang, Jian
Format: Article
Language:English
Subjects:
Basic Study
Cell Line
Chromatography, High Pressure Liquid
Discriminant Analysis
Hepatic Stellate Cells - metabolism
Humans
Least-Squares Analysis
Lipid Metabolism
Metabolic Networks and Pathways
Metabolome
Metabolomics - methods
Principal Component Analysis
Spectrometry, Mass, Electrospray Ionization
Sphingolipids - metabolism
Taurine - metabolism
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Summary:AIM To develop a reliable and simple method to identify important biological metabolites and relevant pathways for taurine in hepatic stellate cells(HSCs), in order to provide more data for taurine therapy.METHODS All the biological samples were analyzed by using highperformance liquid chromatography-time electrospray ionization/quadrupole-time of flight mass spectrometry. Principal component analysis and partial least squares discriminant analysis were used to identify statistically different metabolites for taurine in HSCs, and metabolomic pathway analysis was used to do pathway analysis for taurine in HSCs. The chemical structure of the related metabolites and pathways was identified by comparing the m/z ratio and ion mode with the data obtained from free online databases.RESULTS A total of 32 significant differential endogenous metabolites were identified, which may be related to the mechanism of action of taurine in HSCs. Among the seven relevant pathways identified, sphingolipid metabolism pathway, glutathione metabolism pathway and thiamine metabolism pathway were found to be the most important metabolic pathways for taurine in HSCs.CONCLUSION This study showed that there were distinct changes in biological metabolites of taurine in HSCs and three differential metabolic pathways including sphingolipid pathway, glutathione pathway and thiamine metabolism pathway might be of key importance in mediating the mechanism of action of taurine in HSCs.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v23.i31.5713