High-sensitivity detection of cardiac troponin I with UV LED excitation for use in point-of-care immunoassay

High-sensitivity cardiac troponin assay development enables determination of biological variation in healthy populations, more accurate interpretation of clinical results and points towards earlier diagnosis and rule-out of acute myocardial infarction. In this paper, we report on preliminary tests o...

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Bibliographic Details
Published in:Biomedical optics express 2017-08, Vol.8 (8), p.3749-3762
Main Authors: Rodenko, Olga, Eriksson, Susann, Tidemand-Lichtenberg, Peter, Troldborg, Carl Peder, Fodgaard, Henrik, van Os, Sylvana, Pedersen, Christian
Format: Article
Language:English
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Summary:High-sensitivity cardiac troponin assay development enables determination of biological variation in healthy populations, more accurate interpretation of clinical results and points towards earlier diagnosis and rule-out of acute myocardial infarction. In this paper, we report on preliminary tests of an immunoassay analyzer employing an optimized LED excitation to measure on a standard troponin I and a novel research high-sensitivity troponin I assay. The limit of detection is improved by factor of 5 for standard troponin I and by factor of 3 for a research high-sensitivity troponin I assay, compared to the flash lamp excitation. The obtained limit of detection was 0.22 ng/L measured on plasma with the research high-sensitivity troponin I assay and 1.9 ng/L measured on tris-saline-azide buffer containing bovine serum albumin with the standard troponin I assay. We discuss the optimization of time-resolved detection of lanthanide fluorescence based on the time constants of the system and analyze the background and noise sources in a heterogeneous fluoroimmunoassay. We determine the limiting factors and their impact on the measurement performance. The suggested model can be generally applied to fluoroimmunoassays employing the dry-cup concept.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.8.003749