Transplantation of placenta-derived mesenchymal stem cells reduces hypoxic-ischemic brain damage in rats by ameliorating the inflammatory response

Hypoxic-ischemic brain damage (HIBD) is a common cause of infant death. The purpose of our research was to explore the immunoregulatory mechanism of placenta-derived mesenchymal stem cells (PD-MSCs) in HIBD treatment. Seven-day-old rat pups were randomly divided into HIBD, PD-MSC, fibroblast, and co...

Full description

Saved in:
Bibliographic Details
Published in:Cellular & molecular immunology 2017-08, Vol.14 (8), p.693-701
Main Authors: Ding, Hongfang, Zhang, Hui, Ding, Huifang, Li, Dong, Yi, Xinhao, Ma, Xiaoxu, Li, Ruijuan, Huang, Mei, Ju, Xiuli
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antibodies
Behavior Rating Scale
Biomedical and Life Sciences
Biomedicine
Brain damage
Brain injury
Brain stem
Cytokines
Cytokines - metabolism
Enzyme-linked immunosorbent assay
Female
Fibroblasts
Flow cytometry
Forkhead protein
Forkhead Transcription Factors - metabolism
Foxp3 protein
Gene Expression Regulation
Humans
Hypoxia
Hypoxia - immunology
Hypoxia - therapy
Hypoxia, Brain - immunology
Hypoxia, Brain - therapy
Immunology
Immunoregulation
Immunosuppression
Infant
Inflammation
Inflammation - immunology
Inflammation - therapy
Interleukin 10
Interleukin 17
Ischemia
Ischemia - immunology
Ischemia - therapy
Lymphocytes T
Medical Microbiology
Mesenchymal Stem Cell Transplantation
Mesenchymal stem cells
Mesenchymal Stromal Cells - physiology
Mesenchyme
Microbiology
Neuroprotection
Peripheral blood
Placenta
Placenta - cytology
Polymerase chain reaction
Pregnancy
Rats
research-article
Rodents
Spleen
Stem cell transplantation
Stem cells
T-Lymphocytes, Regulatory - immunology
Tumor necrosis factor-α
Vaccine
γ-Interferon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hypoxic-ischemic brain damage (HIBD) is a common cause of infant death. The purpose of our research was to explore the immunoregulatory mechanism of placenta-derived mesenchymal stem cells (PD-MSCs) in HIBD treatment. Seven-day-old rat pups were randomly divided into HIBD, PD-MSC, fibroblast, and control groups. Forty-eight hours after HIBD induction, cells at a density of 5 × 104 cells/10 µl were injected into the cerebral tissue in the PD-MSC and fibroblast groups. The TNF-α, interleukin- 17 (IL-17), interferon-γ (IFN-γ), and IL-10 levels were detected through quantitative real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Regulatory T cell (Tregs) populations were detected through flow cytometry, and forkhead box P3 (Foxp3) was measured through western blot analysis. Behavioral tests and gross and pathological examinations showed that PD-MSC treatment exerted significantly stronger neuroprotective effects than the other treatments. The expression levels of pro-inflammatory cytokines were substantially upregulated after HI injury. Compared with fibroblast treatment, PD-MSC treatment inhibited the production of pro-inflammatory cytokines and increased the production of IL-10 in the ischemic hemispheres and peripheral blood serum (all P < 0.01). Flow cytometry results showed a notable increase in the number of Tregs within the spleen of the HIBD group. Moreover, the number of Tregs and the Foxp3 expression levels were higher in the PD-MSC treatment group than in the HIBD and fibroblast groups (all P < 0.01). Our research suggests that the mechanism of PD-MSC treatment for HIBD partially involves inflammatory response suppression.
ISSN:1672-7681
2042-0226
DOI:10.1038/cmi.2015.99