Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9

[Display omitted] CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding...

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Bibliographic Details
Published in:Molecular therapy 2017-08, Vol.25 (8), p.1736-1738
Main Authors: Adikusuma, Fatwa, Williams, Nicole, Grutzner, Frank, Hughes, James, Thomas, Paul
Format: Article
Language:English
Subjects:
Animals
Chromosome Deletion
Chromosomes
CRISPR
CRISPR-Cas Systems
Embryonic Stem Cells - metabolism
Females
Gene deletion
Gene Editing
Gene Targeting
Genomes
Letter to the Editor
Males
Mice
RNA, Guide, CRISPR-Cas Systems
Statistical analysis
Stem cells
Zygote
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Description
Summary:[Display omitted] CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.
ISSN:1525-0016
1525-0024
DOI:10.1016/j.ymthe.2017.05.021