Wound Healing Activity of Azadirachta indica A. Juss Stem Bark in Mice

The paste of stem bark of (AI) has been traditionally used on wound and scar for rapid healing in Bundelkhand region of India. In the present investigation, wound healing potential of different extracts of stem bark of AI was explored in mice model. To study the wound healing properties in small ani...

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Bibliographic Details
Published in:Pharmacognosy Magazine 2017-07, Vol.13 (Suppl 2), p.S316-S320
Main Authors: Maan, Preeti, Yadav, Kuldeep Singh, Yadav, Narayan Prasad
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
Diabetes
DNA
Ethanol
Herbal medicine
Laboratories
Leprosy
Original
Plant sciences
Proteins
Rodents
Scars
Seeds
Trees
Ulcers
Worms
Wound healing
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Summary:The paste of stem bark of (AI) has been traditionally used on wound and scar for rapid healing in Bundelkhand region of India. In the present investigation, wound healing potential of different extracts of stem bark of AI was explored in mice model. To study the wound healing properties in small animal model, the excision and incision wound models were used and water, ethanol-water (1:1, v/v) and ethanol extracts were applied topically (15% w/w in ointment base). In the excision wound model, wound contraction, hydroxyproline content, DNA content, protein content, and nitric oxide levels were estimated after 14 days of topical treatment along with histopathological examinations. In the incision wound model, wound breaking strength was determined after 10 days of topical application of different extracts of AI. The animals treated with water extract of AI exhibited significant increment in rate of wound contraction (93.39%, < 0.01), hydroxyproline content (13.31 ± 6.65 mg/g of dry tissue, < 0.001), DNA content (20.99 ± 0.68 μg/100 mg of tissue, < 0.01), protein content (100.53 ± 7.88 mg/g of wet tissue, < 0.01) and nitric oxide level (3.05 ± 0.03 mMol/g of tissue, < 0.001) as well as in wound breaking strength (289.40 ± 29.45 g, < 0.01) when compared with vehicle control group which was also supported by histopathological studies. The water extract of stem bark of AI possesses significant wound healing property, validating its traditional use.
ISSN:0973-1296
0976-4062
DOI:10.4103/0973-1296.210163