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Selective metastatic tumor labeling with green fluorescent protein and killing by systemic administration of telomerase-dependent adenoviruses
We previously constructed telomerase-dependent, replication-selective adenoviruses OBP-301 (Telomelysin) and OBP-401 [Telomelysin-green fluorescent protein (GFP); TelomeScan], the replication of which is regulated by the human telomerase reverse transcriptase promoter. By intratumoral injection, the...
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Published in: | Molecular cancer therapeutics 2009-11, Vol.8 (11), p.3001-3008 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We previously constructed telomerase-dependent, replication-selective adenoviruses OBP-301 (Telomelysin) and OBP-401 [Telomelysin-green
fluorescent protein (GFP); TelomeScan], the replication of which is regulated by the human telomerase reverse transcriptase
promoter. By intratumoral injection, these viruses could replicate within the primary tumor and subsequent lymph node metastasis.
The aim of the present study was to evaluate the possibility of systemic administration of these telomerase-dependent adenoviruses.
We assessed the antitumor efficacy of OBP-301 and the ability of OBP-401 to deliver GFP in hepatocellular carcinoma (HCC)
and metastatic colon cancer nude mouse models. We showed that i.v. administration of OBP-301 significantly inhibited colon
cancer liver metastases and orthotopically implanted HCC. Further, we showed that OBP-401 could visualize liver metastases
by tumor-specific expression of the GFP gene after portal venous or i.v. administration. Thus, systemic administration of these adenoviral vectors should have clinical
potential to treat and detect liver metastasis and HCC. [Mol Cancer Ther 2009;8(11):3001–8] |
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ISSN: | 1535-7163 1538-8514 |
DOI: | 10.1158/1535-7163.MCT-09-0556 |