Unusual Escherichia coli PBP 3 Insertion Sequence Identified from a Collection of Carbapenem-Resistant Enterobacteriaceae Tested In Vitro with a Combination of Ceftazidime-, Ceftaroline-, or Aztreonam-Avibactam

Carbapenemase-producing isolates ( = 110) from health care centers in central Indiana (from 2010 to 2013) were tested for susceptibility to combinations of avibactam (4 μg/ml) with ceftazidime, ceftaroline, or aztreonam. MIC /MIC values were 1/2 μg/ml (ceftazidime-avibactam), 0.5/2 μg/ml (ceftarolin...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2017-08, Vol.61 (8)
Main Authors: Zhang, Yunliang, Kashikar, Ankita, Brown, C Adam, Denys, Gerald, Bush, Karen
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Azabicyclo Compounds
Azabicyclo Compounds - pharmacology
Aztreonam
Aztreonam - pharmacology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
beta-Lactamases - genetics
beta-Lactamases - metabolism
Carbapenem-Resistant Enterobacteriaceae
Carbapenem-Resistant Enterobacteriaceae - drug effects
Carbapenem-Resistant Enterobacteriaceae - genetics
Ceftaroline
Ceftazidime
Ceftazidime - pharmacology
Cephalosporins
Cephalosporins - pharmacology
DNA Transposable Elements - genetics
Drug Combinations
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli Proteins
Escherichia coli Proteins - genetics
Gene Duplication - genetics
Humans
Mechanisms of Resistance
Penicillin-Binding Proteins
Penicillin-Binding Proteins - genetics
Peptidoglycan Glycosyltransferase
Peptidoglycan Glycosyltransferase - genetics
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Summary:Carbapenemase-producing isolates ( = 110) from health care centers in central Indiana (from 2010 to 2013) were tested for susceptibility to combinations of avibactam (4 μg/ml) with ceftazidime, ceftaroline, or aztreonam. MIC /MIC values were 1/2 μg/ml (ceftazidime-avibactam), 0.5/2 μg/ml (ceftaroline-avibactam), and 0.25/0.5 μg/ml (aztreonam-avibactam.) A β-lactam MIC of 8 μg/ml was reported for the three combinations against one isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00389-17