In Vitro Cytotoxicity of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex

The aim of the present study was to evaluate the cytotoxicity of different sealers including GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex on L929 murine fibroblasts. Samples of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex were fabricated in Teflon disks of 5 mm diameter and 3...

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Bibliographic Details
Published in:Iranian endodontic journal 2017, Vol.12 (3), p.354-359
Main Authors: Saygili, Gokhan, Saygili, Suna, Tuglu, Ibrahim, Davut Capar, Ismail
Format: Article
Language:English
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Summary:The aim of the present study was to evaluate the cytotoxicity of different sealers including GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex on L929 murine fibroblasts. Samples of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex were fabricated in Teflon disks of 5 mm diameter and 3 mm thickness. L929 fibroblasts were exposed to the extracts of these materials for 3, 24, 72 and 168 h at 37 C with 5% CO . Cell viability was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The data were analysed by ANOVA. GuttaFlow Bioseal was nontoxic at all experimental time points ( >0.05), whereas MTA Fillapex and AH-Plus were toxic (
ISSN:1735-7497
2008-2746
DOI:10.22037/iej.v12i3.15415