Quantitative identification of senescent cells in aging and disease

Summary Senescent cells are present in premalignant lesions and sites of tissue damage and accumulate in tissues with age. In vivo identification, quantification and characterization of senescent cells are challenging tasks that limit our understanding of the role of senescent cells in diseases and...

Full description

Saved in:
Bibliographic Details
Published in:Aging cell 2017-08, Vol.16 (4), p.661-671
Main Authors: Biran, Anat, Zada, Lior, Abou Karam, Paula, Vadai, Ezra, Roitman, Lior, Ovadya, Yossi, Porat, Ziv, Krizhanovsky, Valery
Format: Article
Language:English
Subjects:
Aging
Aging - genetics
Aging - metabolism
Aging - pathology
Animals
beta-Galactosidase - genetics
beta-Galactosidase - metabolism
Biomarkers - analysis
cancer
cellular senescence
Cellular Senescence - drug effects
Cellular Senescence - genetics
Epithelial Cells - metabolism
Epithelial Cells - pathology
Etoposide - pharmacology
Fibrosis
Flow Cytometry
Gene Expression
Histones - genetics
Histones - metabolism
HMGB1 Protein - genetics
HMGB1 Protein - metabolism
Humans
Image processing
Image Processing, Computer-Assisted
ImageStreamX
Lymphocytes - metabolism
Lymphocytes - pathology
Mice
Molecular Imaging
Neoplasms - genetics
Neoplasms - metabolism
Neoplasms - pathology
Original
Primary Cell Culture
Senescence
senescence‐associated beta‐galactosidase
Single-Cell Analysis - methods
Staining and Labeling - methods
Stromal Cells - metabolism
Stromal Cells - pathology
β-Galactosidase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Senescent cells are present in premalignant lesions and sites of tissue damage and accumulate in tissues with age. In vivo identification, quantification and characterization of senescent cells are challenging tasks that limit our understanding of the role of senescent cells in diseases and aging. Here, we present a new way to precisely quantify and identify senescent cells in tissues on a single‐cell basis. The method combines a senescence‐associated beta‐galactosidase assay with staining of molecular markers for cellular senescence and of cellular identity. By utilizing technology that combines flow cytometry with high‐content image analysis, we were able to quantify senescent cells in tumors, fibrotic tissues, and tissues of aged mice. Our approach also yielded the finding that senescent cells in tissues of aged mice are larger than nonsenescent cells. Thus, this method provides a basis for quantitative assessment of senescent cells and it offers proof of principle for combination of different markers of senescence. It paves the way for screening of senescent cells for identification of new senescence biomarkers, genes that bypass senescence or senolytic compounds that eliminate senescent cells, thus enabling a deeper understanding of the senescent state in vivo.
ISSN:1474-9718
1474-9726
DOI:10.1111/acel.12592