Near-infrared fluorescence activation probes based on disassembly-induced emission cyanine dye† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc01330e Click here for additional data file

In the presence of target analyte, bright fluorescence in the near-IR region is emitted through the recognition-induced disassembly of the probe aggregate. Currently most of the fluorogenic probes are designed for the detection of enzymes which work by converting the non-fluorescence substrate into...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2015-05, Vol.6 (8), p.4643-4649
Main Authors: Hou, Tai-Cheng, Wu, Ying-Yi, Chiang, Po-Yi, Tan, Kui-Thong
Format: Article
Language:English
Subjects:
Chemistry
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Summary:In the presence of target analyte, bright fluorescence in the near-IR region is emitted through the recognition-induced disassembly of the probe aggregate. Currently most of the fluorogenic probes are designed for the detection of enzymes which work by converting the non-fluorescence substrate into the fluorescence product via an enzymatic reaction. On the other hand, the design of fluorogenic probes for non-enzymatic proteins remains a great challenge. Herein, we report a general strategy to create near-IR fluorogenic probes, where a small molecule ligand is conjugated to a novel γ-phenyl-substituted Cy5 fluorophore, for the selective detection of proteins through a non-enzymatic process. Detail mechanistic studies reveal that the probes self-assemble to form fluorescence-quenched J-type aggregate. In the presence of target analyte, bright fluorescence in the near-IR region is emitted through the recognition-induced disassembly of the probe aggregate. This Cy5 fluorophore is a unique self-assembly/disassembly dye as it gives remarkable fluorescence enhancement. Based on the same design, three different fluorogenic probes were constructed and one of them was applied for the no-wash imaging of tumor cells for the detection of hypoxia-induced cancer-specific biomarker, transmembrane-type carbonic anhydrase IX.
ISSN:2041-6520
2041-6539
DOI:10.1039/c5sc01330e