Genome-wide changes in microRNA expression during short and prolonged heat stress and recovery in contrasting rice cultivars

MicroRNAs (miRNAs) are known to regulate expression of genes under stress. We report here the deep sequencing of small RNAs expressed during control, short and prolonged heat stress and recovery. Genome-wide identification of miRNAs in tolerant (Nagina 22) and susceptible (Vandana) rice cultivars wa...

Full description

Saved in:
Bibliographic Details
Published in:Journal of experimental botany 2017-04, Vol.68 (9), p.2399-2412
Main Authors: Mangrauthia, Satendra K., Bhogireddy, Sailaja, Agarwal, Surekha, Prasanth, Vishnu V., Voleti, S. R., Neelamraju, Sarla, Subrahmanyam, Desiraju
Format: Article
Language:English
Subjects:
Gene Expression Regulation, Plant
Genome, Plant
High-Throughput Nucleotide Sequencing
Hot Temperature
MicroRNAs - genetics
Oryza - genetics
Plant-Environment Interactions
Research Paper
RNA, Plant - genetics
Seedlings - genetics
Sequence Analysis, RNA
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) are known to regulate expression of genes under stress. We report here the deep sequencing of small RNAs expressed during control, short and prolonged heat stress and recovery. Genome-wide identification of miRNAs in tolerant (Nagina 22) and susceptible (Vandana) rice cultivars was performed in 16 samples representing root and shoot of 13-day-old seedlings. The expression profile of miRNAs was analysed in 36 pairwise combinations to identify the genotype-, treatment- and tissue-dependent expression of miRNAs. Small-RNA sequencing of 16 libraries yielded ~271 million high-quality raw sequences; 162 miRNA families were identified. The highly expressed miRNAs in rice tissues were miR166, miR168, miR1425, miR529, mR162, miR1876, and miR1862. Expression of osa-miR1436, osa-miR5076, osa-miR5161, and osa-miR6253 was observed only in stressed tissue of both genotypes indicating their general role in heat stress response. Expression of osa-miR1439, osa-miR1848, osa-miR2096, osa-miR2106, osa-miR2875, osa-miR3981, osa-miR5079, osa-miR5151, osa-miR5484, osa-miR5792, and osa-miR5812 was observed only in Nagina 22 during high temperature, suggesting a specific role of these miRNAs in heat stress tolerance. This study provides details of the repertoire of miRNAs expressed in root and shoot of heat susceptible and tolerant rice genotypes under heat stress and recovery.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erx111