Loading…
In Vitro Binding of Agrobacterium tumefaciens to Plant Cells from Suspension Culture 1
In vitro binding experiments were carried out using 32 P-labeled cells of the virulent Agrobacterium tumefaciens strain B6 and Datura innoxia cells from suspension culture. Binding kinetics showed that adherence of bacteria to Datura cells increased gradually during the first 60 minutes and attained...
Saved in:
Published in: | Plant physiology (Bethesda) 1979-02, Vol.63 (2), p.382-387 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | In vitro
binding experiments were carried out using
32
P-labeled cells of the virulent
Agrobacterium tumefaciens
strain B6 and
Datura innoxia
cells from suspension culture. Binding kinetics showed that adherence of bacteria to
Datura
cells increased gradually during the first 60 minutes and attained a maximum level within 120 minutes of incubation. Maximum binding occurred at pH 6.0. The presence of Ca
2+
and Mg
2+
reduced binding slightly and EDTA had little effect at concentrations of 0.1 to 10 millimolar. The binding of bacteria to
Datura
cells was temperature-dependent.
Escherichia coli, Salmonella typhimurium, Rhizobium japonicum
, and
Micrococcus lysodeikticus
did not compete with virulent
A. tumefaciens
strain B6 for binding to
Datura
cells. The admixture of avirulent
A. tumefaciens
strain IIBNV6 enhanced adherence of virulent
A. tumefaciens
strain B6 to
Datura
cells. Octopine had no effect on the binding of virulent
A. tumefaciens
strain B6 to
Datura
cells, but 10 millimolar canavanine was inhibitory. Arginine enhanced the adherence of the bacteria at concentrations higher than 0.1 millimolar. Incubation with DNase, RNase, and lipase did not affect the binding, but protease stimulated the adherence of bacteria to
Datura
cells. Concanavaline A and soybean lectin had little effect whereas lecithin and lysolecithin enhanced binding slightly. Poly-
l
-lysine markedly stimulated the bacteria-plant cell adherence. Cells from suspension cultures of pea, vetch, and soybean had a 2- to 3-fold higher binding capacity than
Datura
cells, whereas cells from wheat, corn, rice, and sorghum had a considerably lower affinity for binding with virulent
A. tumefaciens
strain B6. Bacterial adherence to plant cells was confirmed by autoradiography and electron microscopy. Autoradiographic analysis showed that bacteria were associated with the cell wall, and that often binding of bacteria was localized. Electron micrographs clearly illustrated a tight association of virulent
A. tumefaciens
strain B6 cells to the
Datura
cell wall. |
---|---|
ISSN: | 0032-0889 1532-2548 |