The TLR7 agonist imiquimod induces anti-cancer effects via autophagic cell death and enhances anti-tumoral and systemic immunity during radiotherapy for melanoma

Toll-like receptor (TLR) ligands are strongly considered immune-adjuvants for cancer immunotherapy and have been shown to exert direct anti-cancer effects. This study was performed to evaluate the synergistic anti-cancer and anti-metastatic effects of the TLR7 agonist imiquimod (IMQ) during radiothe...

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Bibliographic Details
Published in:Oncotarget 2017-04, Vol.8 (15), p.24932-24948
Main Authors: Cho, Jeong Hyun, Lee, Hyo-Ji, Ko, Hyun-Jeong, Yoon, Byung-Il, Choe, Jongseon, Kim, Keun-Cheol, Hahn, Tae-Wook, Han, Jeong A, Choi, Sun Shim, Jung, Young Mee, Lee, Kee-Ho, Lee, Yun-Sil, Jung, Yu-Jin
Format: Article
Language:English
Subjects:
Aminoquinolines - pharmacology
Animals
Antineoplastic Agents - pharmacology
Autophagy - drug effects
Cell Death - drug effects
Chemoradiotherapy
Disease Models, Animal
Melanoma, Experimental - drug therapy
Melanoma, Experimental - immunology
Melanoma, Experimental - pathology
Melanoma, Experimental - radiotherapy
Membrane Glycoproteins - agonists
Mice
Mice, Inbred C57BL
Research Paper
Toll-Like Receptor 7 - agonists
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Summary:Toll-like receptor (TLR) ligands are strongly considered immune-adjuvants for cancer immunotherapy and have been shown to exert direct anti-cancer effects. This study was performed to evaluate the synergistic anti-cancer and anti-metastatic effects of the TLR7 agonist imiquimod (IMQ) during radiotherapy for melanoma. The pretreatment of B16F10 or B16F1 cells with IMQ combined with γ-ionizing radiation (IR) led to enhanced cell death via autophagy, as demonstrated by increased expression levels of autophagy-related genes, and an increased number of autophagosomes in both cell lines. The results also confirmed that the autophagy process was accelerated via the reactive oxygen species (ROS)-mediated MAPK and NF-κB signaling pathway in the cells pretreated with IMQ combined with IR. Mice subcutaneously injected with melanoma cells showed a reduced tumor growth rate after treatment with IMQ and IR. Treatment with 3-methyladenine (3-MA), ameliorated the anti-cancer effect of IMQ combined with IR. Additionally, the combination therapy enhanced anti-cancer immunity, as demonstrated by an increased number of CD8+ T cells and decreased numbers of regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSCs) in the tumor lesions. Moreover, the combination therapy decreased the number of metastatic nodules in the lungs of mice that were injected with B16F10 cells via the tail vein. In addition, the combination therapy enhanced systemic anti-cancer immunity by increasing the abundances of T cell populations expressing IFN-γ and TNF-α. Therefore, these findings suggest that IMQ could serve as a radiosensitizer and immune booster during radiotherapy for melanoma patients.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.15326