Psychosine variants as antigens for natural killer T cells† †Electronic supplementary information (ESI) available: Experimental details for synthesis of glycolipids shown in Fig. 3, cytokine quantification, and NKT cell percentage measurements. GM-SCF release from human NKT cell lines stimulated by alpha-galactosylceramide. Cytokine release (plasma) from mice injected with alpha-Glc-psychosine (phyto) and alpha-psychosine (phyto). See DOI: 10.1039/c6sc04218j Click here for additional data file

Truncated forms of alpha-glycosylceramides are antigens for natural killer T cells. Natural killer T (NKT) cells play a central role in the interface between innate and adaptive immunity, and alpha-galactosylceramide was recently shown to be an endogenous antigen for these cells. The source of alpha...

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Published in:Chemical science (Cambridge) 2016-12, Vol.8 (3), p.2204-2208
Main Authors: Deng, S., Kain, L., Pereira, C. S., Mata, S., Macedo, M. F., Bendelac, A., Teyton, L., Savage, P. B.
Format: Article
Language:English
Subjects:
Chemistry
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Summary:Truncated forms of alpha-glycosylceramides are antigens for natural killer T cells. Natural killer T (NKT) cells play a central role in the interface between innate and adaptive immunity, and alpha-galactosylceramide was recently shown to be an endogenous antigen for these cells. The source of alpha-galactosylceramide has not yet been determined; however, in vivo degradation of alpha-galactosylceramide involves generation of alpha-psychosine (alpha-galactosylsphingosine). Alpha-psychosine stimulates cytokine release from NKT cells and constitutes an endogenous antigen for these cells. Alpha-psychosine contains a single lipid chain, while most antigens for NKT cells have two lipid chains, and we have investigated if other glycolipids with one lipid chain, derived from know antigens for NKT cells, stimulate cytokine release from NKT cells. Only psychosine variants derived from the most potent NKT cell antigens cause stimulation, and this stimulation occurs in vitro as well as in vivo . Truncated forms of weak antigens for NKT cells are not stimulatory.
ISSN:2041-6520
2041-6539
DOI:10.1039/c6sc04218j