Differential processing of small RNAs during endoplasmic reticulum stress

The accumulation of misfolded proteins in the endoplasmic reticulum (ER) lumen due to the disruption of the homeostatic system of the ER leads to the induction of the ER stress response. Cellular stress-induced pathways globally transform genes expression on both the transcriptional and post-transcr...

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Bibliographic Details
Published in:Scientific reports 2017-04, Vol.7 (1), p.46080-46080, Article 46080
Main Authors: Mesitov, Mikhail V, Soldatov, Ruslan A, Zaichenko, Danila M, Malakho, Sophie G, Klementyeva, Tatyana S, Sokolovskaya, Alisa A, Kubatiev, Aslan A, Mironov, Andrey A, Moskovtsev, Aleksey A
Format: Article
Language:English
Subjects:
Angiogenin
Base Sequence
Cellular stress response
Dithiothreitol - pharmacology
Endoplasmic reticulum
Endoplasmic Reticulum Stress - drug effects
Endoplasmic Reticulum Stress - genetics
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic - drug effects
Gene Library
Humans
Isoforms
Isotypes
Jurkat Cells
Lymphocytes T
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Molecular Sequence Annotation
Nucleic Acid Conformation
Nucleotides - genetics
Post-transcription
Protein folding
Ribonuclease
RNA processing
RNA Processing, Post-Transcriptional - drug effects
RNA Processing, Post-Transcriptional - genetics
RNA, Transfer - chemistry
RNA, Transfer - genetics
RNA, Transfer - metabolism
Stress response
T-Lymphocytes - drug effects
T-Lymphocytes - metabolism
Transcription
Transcriptome - drug effects
Transcriptome - genetics
tRNA
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Summary:The accumulation of misfolded proteins in the endoplasmic reticulum (ER) lumen due to the disruption of the homeostatic system of the ER leads to the induction of the ER stress response. Cellular stress-induced pathways globally transform genes expression on both the transcriptional and post-transcriptional levels with small RNA involvement as regulators of the stress response. The modulation of small RNA processing might represent an additional layer of a complex stress response program. However, it is poorly understood. Here, we studied changes in expression and small RNAs processing upon ER stress in Jurkat T-cells. Induced by ER-stress, depletion of miRNAs among small RNA composition was accompanied by a global decrease of 3' mono-adenylated, mono-cytodinylated and a global increase of 3' mono-uridinylated miRNA isoforms. We observed the specific subset of differentially expressed microRNAs, and also the dramatic induction of 32-nt tRNA fragments precisely phased to 5' and 3' ends of tRNA from a subset of tRNA isotypes. The induction of these tRNA fragments was linked to Angiogenin RNase, which mediates translation inhibition. Overall, the global perturbations of the expression and processing of miRNAs and tiRNAs were the most prominent features of small RNA transcriptome changes upon ER stress.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep46080