Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing

Summary The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 to target the FAD2 gene in Arabidopsis thaliana and in the closely related emerging oil seed plant, Camelina sativa...

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Bibliographic Details
Published in:Plant biotechnology journal 2017-05, Vol.15 (5), p.648-657
Main Authors: Jiang, Wen Zhi, Henry, Isabelle M., Lynagh, Peter G., Comai, Luca, Cahoon, Edgar B., Weeks, Donald P.
Format: Article
Language:English
Subjects:
allohexaploid
Arabidopsis - genetics
Arabidopsis thaliana
Biofuels
Brassicaceae - genetics
Brassicaceae - metabolism
Camelina
Camelina sativa
Composition
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9
Deoxyribonucleic acid
DNA
Enzymes
Experiments
FAD2 gene
Fatty acid composition
Fatty Acid Desaturases - genetics
Fatty acids
Fatty Acids - biosynthesis
Fatty Acids - genetics
Gene Editing
Genes
Genetic engineering
Genetic modification
Genomes
Germ-Line Mutation
Linoleic acid
Linolenic acid
Linolenic acids
Mutation
Nuclease
Oil
Oleic acid
Organic chemistry
Plant Leaves - genetics
Plants, Genetically Modified
Polymerase Chain Reaction
Polyploidy
Polyunsaturated fatty acids
RNA, Guide, CRISPR-Cas Systems
Seeds
Seeds - genetics
Seeds - metabolism
Technology application
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Summary:Summary The CRISPR/Cas9 nuclease system is a powerful and flexible tool for genome editing, and novel applications of this system are being developed rapidly. Here, we used CRISPR/Cas9 to target the FAD2 gene in Arabidopsis thaliana and in the closely related emerging oil seed plant, Camelina sativa, with the goal of improving seed oil composition. We successfully obtained Camelina seeds in which oleic acid content was increased from 16% to over 50% of the fatty acid composition. These increases were associated with significant decreases in the less desirable polyunsaturated fatty acids, linoleic acid (i.e. a decrease from ~16% to
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.12663