Toxicity of ZnO and TiO2 to Escherichia coli cells

Abstract We performed a comprehensive investigation of the toxicity of ZnO and TiO 2 nanoparticles using Escherichia coli as a model organism. Both materials are wide band gap n-type semiconductors and they can interact with lipopolysaccharide molecules present in the outer membrane of E. coli , as...

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Bibliographic Details
Published in:Scientific reports 2016-10, Vol.6 (1), p.35243, Article 35243
Main Authors: Leung, Yu Hang, Xu, Xiaoying, Ma, Angel P. Y., Liu, Fangzhou, Ng, Alan M. C., Shen, Zhiyong, Gethings, Lee A., Guo, Mu Yao, Djurišić, Aleksandra B., Lee, Patrick K. H., Lee, Hung Kay, Chan, Wai Kin, Leung, Frederick C. C.
Format: Article
Language:English
Subjects:
Antibacterial activity
E coli
Escherichia coli
Illumination
Lipid peroxidation
Lipopolysaccharides
Nanomaterials
Nanoparticles
Nanotechnology
Oxidative stress
Oxides
Proteins
Reactive oxygen species
Toxicity
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Summary:Abstract We performed a comprehensive investigation of the toxicity of ZnO and TiO 2 nanoparticles using Escherichia coli as a model organism. Both materials are wide band gap n-type semiconductors and they can interact with lipopolysaccharide molecules present in the outer membrane of E. coli , as well as produce reactive oxygen species (ROS) under UV illumination. Despite the similarities in their properties, the response of the bacteria to the two nanomaterials was fundamentally different. When the ROS generation is observed, the toxicity of nanomaterial is commonly attributed to oxidative stress and cell membrane damage caused by lipid peroxidation. However, we found that significant toxicity does not necessarily correlate with up-regulation of ROS-related proteins. TiO 2 exhibited significant antibacterial activity, but the protein expression profile of bacteria exposed to TiO 2 was different compared to H 2 O 2 and the ROS-related proteins were not strongly expressed. On the other hand, ZnO exhibited lower antibacterial activity compared to TiO 2 , and the bacterial response involved up-regulating ROS-related proteins similar to the bacterial response to the exposure to H 2 O 2 . Reasons for the observed differences in toxicity and bacterial response to the two metal oxides are discussed.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep35243