Gabapentin Attenuates Ocular Inflammation: In vitro and In vivo Studies

To investigate the effects of gabapentin, a structural analog of γ-amino butyric acid (GABA), on the inflammatory response of lipopolysaccharide (LPS)-stimulated rabbit corneal cells (SIRC) and on endotoxin-induced uveitis (EIU) in rabbits. We investigated the LPS-induced expression of several infla...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in pharmacology 2017-04, Vol.8, p.173-173
Main Authors: Anfuso, Carmelina D, Olivieri, Melania, Fidilio, Annamaria, Lupo, Gabriella, Rusciano, Dario, Pezzino, Salvatore, Gagliano, Caterina, Drago, Filippo, Bucolo, Claudio
Format: Article
Language:English
Subjects:
Pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate the effects of gabapentin, a structural analog of γ-amino butyric acid (GABA), on the inflammatory response of lipopolysaccharide (LPS)-stimulated rabbit corneal cells (SIRC) and on endotoxin-induced uveitis (EIU) in rabbits. We investigated the LPS-induced expression of several inflammatory mediators, such as TNF-α, IL-1β, cPLA , COX-2, and PGE in the SIRC cells with or without gabapentin treatment. Gabapentin treatment significantly ( < 0.05) attenuated cytokines production, cPLA activation, COX-2 expression, and PGE levels in SIRC. EIU was induced by an intraocular injection of 0.1 μg of LPS in albino rabbit eye. After 7 and 24 h from LPS injection clinical signs of ocular inflammation were examined by slit lamp with or without topical treatment of 0.5% gabapentin. Tears, aqueous, cornea, conjunctiva, and iris-ciliary body were collected and inflammatory biomarkers assessed. Topical treatment with gabapentin significantly ( < 0.05) reduced clinical signs and biomarkers of inflammation compared with the LPS group both at 7 and 24 h. In conclusion, the results generated in the present study suggest that ophthalmic formulation based on gabapentin may be useful in the treatment of inflammatory conditions associated to ocular pain such as uveitis, and that clinical studies to evaluate this possibility may be warranted.
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2017.00173